The following gel shows the results of a crime scene investigation. Lane 1 shows a DNA sample that was obtained from the scene of a crime (there was evidence of the criminal cutting himself, so it's the criminal's blood sample that produced the DNA bands in lane 1). Lanes 2-7 shows a DNA sample from six potential suspects held in connection with the crime. Arrange the bands labeled A, B, C, D from largest to smallest. 1 2 3 4 5 6 7 OC, A, D, B A, B, C, D OB, D, A, C O D, A, C, B
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- The Evidence shows which suspect was at the crime scene? ( image of murder investigation) A) suspect A B) suspect B C) suspect C D) none were at the crime scene Which of the following choices most likely lists the sizes of the four DNA fragments in lane 6 ( second image) of this agarose gel stained with ethidium bromide? ( Note: the well that the DNA was loaded into is at the top of the gel image). A) 100 bp, 300 bp, 450 bp, 600 bp. B) 300 bp, 400 bp, 100 bp, 600 bp C) 600 bp, 450 bp, 300 bp, 100 bp D) 400 bp, 300 bp, 600 bp, 100 bp E) DNA fragment size can't be estimated using an agarose gel.Refer to the DNA profiles comparing the DNA obtained from the three suspects with the crime-scene DNA. Use a ruler to align the bands found under the crime-scene DNA with any of the bands found in the suspects’ DNA. Place the ruler at the bottom of the bands. Take notes on whether any of the suspects’ DNA shares the same bands as the crime-scene DNA. Analyze your notes on each suspect’s DNA profile. Do any suspects share all the DNA bands found in the evidence DNA?what does DNA tests tell us about the time frame or circumstances under which DNA came to be associated with an evidence sample? It depends on the sample Who was the primary wearer of an article of clothing If the DNA was depositied as a part of the crime or from innocent contact The order in which the individuals contributed DNA to an evidence sample
- Directions: The following story is about a crime solved through biotechnological techniques. The techniques used are DNA fingerprinting and polymerase chain reaction (PCR). DNA fingerprinting is a technique in which an individual’s DNA is analyzed to reveal the pattern of particular short nucleotide sequences. This pattern is claimed to be unique to the individual concerned and can thus be used for identification purposes. Polymerase chain reaction, on the other hand, is a technique used to replicate a DNA fragment so as to produce many copies of a particular DNA sequence. Why do you think there’s a need to generate several copies of the DNA? You will learn the answers to this as you go through the story. Read the story carefully and answer the questions that follow.Directions: The following story is about a crime solved through biotechnological techniques. The techniques used are DNA fingerprinting and polymerase chain reaction (PCR). DNA fingerprinting is a technique in which an individual’s DNA is analyzed to reveal the pattern of particular short nucleotide sequences. This pattern is claimed to be unique to the individual concerned and can thus be used for identification purposes. Polymerase chain reaction, on the other hand, is a technique used to replicate a DNA fragment so as to produce many copies of a particular DNA sequence. Why do you think there’s a need to generate several copies of the DNA? You will learn the answers to this as you go through the story. Read the story carefully and answer the questions that follow. Questions: 1. How did the investigators conclude that the suspect was indeed the murderer? 2. What if a strand of hair was found in the truck bed instead of a seed pod, do you think it will still lead to the…. Lane 1 represents DNA taken from the murdered victim. Lane 2 through 5 are DNA samples taken from suspects in the crime. Lane 6 is from a bloodstain found near the victim. Q6. Why is it important to include the DNA taken from the murdered victim (Lane 1) in this DNA analysis?
- Someone broke into the bookstore and stole thousands of dollars’ worth of textbooks. Some hairs were left behind, from which DNA was extracted. An individual was caught with all five of the stolen textbooks in his bag. He claims his friend gave them to him. You decide to amplify two regions of the DNA (two loci, plural of locus) and digest the samples, and get the following result on the electrophoresis gel.L = Standard marker 1 = Suspect 1 2 = Suspect 2 3 = DNA from crime scene (Note: assume bands that are close in distance traveled are the same length. Higher concentrations of DNA can make a band appear to travel farther.) Which band patterns are similar?Someone broke into the bookstore and stole thousands of dollars’ worth of textbooks. Some hairs were left behind, from which DNA was extracted. An individual was caught with all five of the stolen textbooks in his bag. He claims his friend gave them to him. You decide to amplify two regions of the DNA (two loci, plural of locus) and digest the samples, and get the following result on the electrophoresis gel.L = Standard marker 1 = Suspect 1 2 = Suspect 2 3 = DNA from crime scene (Note: assume bands that are close in distance traveled are the same length. Higher concentrations of DNA can make a band appear to travel farther.) Who is most likely the culprit, and how do you know?The key portion of the autoradiograph from a single locus probe analysis of various DNA samples in a rape investigation is shown in this figure. (Please refer to the attached Picture) Samples of DNA were loaded into the following lanes: (based on the picture) known blood sample of victim known blood sample from defendant DNA size markers female fraction from vaginal swab of victim male fraction from vaginal swab of If you are the DNA analyst, you should conclude that: A. The suspect is guilty B. The suspect might be guilty, but more probes should be used. Explain your answer and Provide Conclusion.
- Suppose a suspicious hair was found in a victim's home. A gel is set up with a DNA ladder in lane 1. The victim's DNA fragments are in lane 2, as a negative control. The DNA fragments of the suspicious hair are in lane 3. The DNA fragments of suspected criminal #1 are in lane 4, and the DNA fragments of suspected criminal # 2 are in lane 5. The resulting gel is below . Which suspect , if any, committed the crime? Explain your answer .This lane contains a 1500 base pair (bp) DNA fragment. A. Lane 1 B. Lane 2 C. Lane 3 D. Lane 4a. DNA fingerprinting is a method of visualizing similarities and differences in the DNA sequence of individuals. Arrange the following items so that they show the DNA fingerprinting steps in proper order: - Gel electrophoresis - Stain or probe for DNA - cut DNA into fragments - Amplify DNA sample using PCR b. On the gel shown below are four DNA samples. Samples A to C are taken from tissues of landslide victims that are being identified, while sample D came from a hair sample brought by a mother looking for the remains of her son. (see img) i. If similar band patterns in a gel are created using the same restriction enzyme, what does that tell you about the DNA sequence of the samples? ii. In sample C, only two fragments were created. How many restriction sites (regions where enzymes cut) are present in sample C?