under certain conditions alpha helical bundles can form and can facilitate protein insertion into membranes. discuss how they coudl achieve this at a biochemical level

Q: For the investigation of positive homotropic cooperativity within protein-ligand binding…

A: When a receptor binds to a ligand and scales in a non-linear fashion with ligand concentration, it…

Q: Some of the following four amino acids : alanine, arginine, histidine, aspartic acid would provide a…

A: Acid-Base Catalysis is involved in the reaction mechanisms that requires the transfer of a proton…

Q: Both hsp60-like and hsp70 molecular chaperonesshare an affinity for exposed hydrophobic patches on…

A: Molecular chaperones are defined as the proteins that aid or help in the folding or unfolding of…

Q: Given the following data, calculate Keq for the denaturation reaction of the protein β-lactoglobin…

A: Free energy change for a reaction, ΔG, can be solved using the standard change in Gibbs free energy,…

Q: What are some examples of biochemical tests for proteins that involve acid hydrolysis or base…

A: Proteins: Proteins are complex high-molecular-weight molecules that consist of amino acids joined…

Q: Both hsp60-like and hsp 70 molecular chaperones share an affinity for exposed hydrophobic patches on…

A: Answer: Molecular chaperones are defined as the proteins that aid or help in the folding or…

Q: proteins are deamidated in an aqueous solution to produce ammonium and aspartate, respectively, from…

A: Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different…

Q: Protein purification table: A 50 ml crude skeletal muscle extract contains 32mg of protein per ml.…

A: Amount of initial extract formed = 0.14 µmol /minute. Protein = 32mg/ml,  given then for 10…

Q: describe a detailed experimental procedure for the chemical synthesis of proteins with the…

A: Proteins are vital biomolecules that plays key role in biochemical pathways. It synthesis occurs in…

Q: Fibrinogen contains tyrosine- O - sulfate. Propose an activated form of sulfate that could react in…

A: Tyrosine O-sulfation of protein is a post-translational modification that mostly occurs in…

Q: Let's consider a protein with two surface-exposed histidine residues: • Hisa is a "typical"…

A: The Henderson-Hasselbalch Equation for the deprotonation of a species is given below. pH = pKa +…

Q: Give the general Adiar equation for the binding of a ligand to a dimeric protein. Explain further…

A: Cooperativity- It is a character of some multi-subunit enzymes, where different subunits has…

Q: Degradation signals are commonly located in protein regions that also facilitate protein–protein…

A: Domains are considered as the structural unit present in the protein subunit, which is responsible…

Q: What is the smallest number of molecules of ATP and GTP consumed in the synthesis of a 200- residue…

A: ATP is adenosine triphosphate and is an organic compound. It is a hydrotrope that provides energy to…

Q: Protein: QQICIMFELTQISS Predict the products of the following reactions with the protein given, if…

A: Introduction The above protein can be decoded by using the one-letter code table. The given proteins…

Q: Why does it make biochemical sense that chaperones recognize hydrophobicsurface area? What…

A: Chaperons are meant to prevent irreversible aggregation of unfolded proteins, which is mediated by…

Q: (A) What property of a protein might make it difficult to transfer it from polyacrylamide gel to…

A: Nitrocellulose membranes are a popular matrix used in protein blotting because of their high…

Q: Draw the pyranose form of D-Glucose as a Haworth projection. Identify your projection as either the…

A: Anhydrous Dextrose is the anhydrous form of D-glucose, which is a natural monosaccharide. Dextrose…

Q: A protein gives, under conditions of buffer composition, pH, and temperaturethat are close to…

A: SDS-PAGE is an electrophoretic technique that separates proteins based on their weight. BME is a…

Q: The amino acid that is often used as a tag (in a run of 6-8 of this amino acid in a row) for protein…

A: Living cells have a complex system for protein synthesis. Ribosomes synthesize amino acid chains.…

Q: Some proteins migrate anomalously in SDS-PAGE gels. For instance, the molecular weight determined…

A: SDS-PAge is the method that separates the proteins on the basis of their molecular weight. It is an…

Q: dentify and describe how you would PEGylate this peptide at its N- terminal amine. Discuss the…

A: The process of PEGylation improves drug solubility and decreases immunogenicity of a molecule that…

Q: In biochemical and structural terms, how does the cellular form of the prion protein differ from the…

A: There are different macromolecules present in the living organism. Of which proteins are one of the…

Q: Bradford Assay is most suitable to use when the extraction buffer is below the  target protein’s pI.…

A: The Bradford assay is a quick and sensitive method for measuring the concentrations of proteins. The…

Q: Equilibrium constants are always dependent upon temperature. In determining equilibrium constants…

A: The equilibrium constant is defined as the number that expresses the relationship between the…

Q: Briefly describe the conditions of protein sample labeling and NMR sample. ?

A: Nuclear magnetic resonance or NMR is used to analyze the structure of a material. It analyzes the…

Q: . (a) Explain the biochemical basis for the fact that one can synchro- nize cell populations by…

A: dATP is a nucleotide used in cells for DNA synthesis, as a substrate of DNA polymerase. It is…

Q: indicate at the structural level how 3TC works as a deoxycytosine analog, and why it is a chain…

A: The synthesis of a DNA molecule from the double stranded DNA template by the enzyme DNA polymerase…

Q: Explain what steps would be suitable to prepare a successful chromatographic peptide mapping?

A: Peptide mapping is used to identify primary structure of protein. Other uses of peptide mapping…

Q: What is the first thing that must occur in order for the catalysis of the peptide bond to begin.…

A: Macromolecules are large organic molecules composed of thousands of covalently connected atoms and…

Q: how are SDS-PAGE and BN-PAGE techniques used determine the composition of protein-photosynthetic…

A: PAGE OR the polyacrylamide gel electrophoresis is a kind of molecular technique that involves the…

Q: Calculate the energy required, in ATP equivalents, to synthesize a 100-residue protein from free…

A: ATP is Adenosine Triphosphate and GTP is Guanosine Triphosphate , in which ATP is used to carry…

Q: Affinity purification: Assemble the following components to set up affinity chromatography to purify…

A: Affinity chromatography: It is a chromatographic technique for the separation of the compound on…

Q: Analysis of a peptide antibiotic purified from a strain of Bacillus brevii resulted in the following…

A: Average mol weight of one amino acid is 110D. The peptide is of 1200D. This means that the peptide…

Q: Approximate molecular weight for an unknown protein from gel-filtration experiment is 130 kDa.…

A: Given Values: Molecular weight obtained from gel-filtration experiment = 130 kDa Weight of the…

Q: In the fungus Neurospora, some mutants were obtainedthat lacked activity for a certain enzyme. The…

A: Neurospora It belongs to the Ascomycete fungus family. The genus name, "nerve spore," refers to the…

Q: Explain the biochemical defect that leads to Lesch-Nyhan syndrome and suggest how the defect might…

A: Introduction: Purines (adenine and guanine) and pyrimidines (cytosine, thymine, and uracil) play…

Q: A family of proteins known as cupredoxins contain a single redoxactive Cu ion coordinated by a Cys,…

A: Proteins are macromolecules or large biomolecules. Protein contains one or more long chains of amino…

Q: Consider a protein with two surface-exposed histidine residues: HisA is a “typical” histidine…

A: Histidine is a basic residue having a amino group in its side chain and involved in the form of…

Q: a) Provide reaction schemes to show two different types of covalent modification in regulating…

A: Covalent modification of protein is the process by which a functional group is covalently linked to…

Q: Describe in detail with the aid of diagrams the use of chemical shift perturbation mapping to…

A: Before getting into protein-ligand binding site mapping using chemical shift perturbations, lets…

Q: . In the protein adenylate kinase, the C-terminal region is a-helical, with the sequence…

A: Protein is a macronutrient that is essential to building muscle mass. It is commonly found in animal…

Q: Shown is the electrophoretic profile of a pure protein ran at different pH values "H H H H (+) (-)…

A: The net charge of a protein depends on the pH of its environment and the net charge determines the…

Q: A protein of unknown structure has been purified. Size-exclusion chromatograp reveals that the…

A: Proteins are made up of a long polypeptide chain, which consists of amino acid residues. Proteins…

Q: The purification continues with a cation exchange step in which the positively charged cytochrome C…

A: A linear XY linear graph for the equation. A straight line "y = mx + b" where y = absorbance at 595…

Q: Some of the following four amino acids : alanine, arginine, histidine, aspartic acid would provide a…

A: Introduction: Acid-base catalysis is a mechanism in which the transfer of a proton from an acid…

Q: Which biochemical processes might be affected by a deficiency of thiamine?

A: The normal function of the human body relies on proper nutrition which can be derived from vitamins…

Q: Cone snails are carnivores that inject a powerful set of toxins into their prey, leading to rapid…

A: A rare species of cone snail carnivoures  is a powerful venom for their prey. Its powerful venom…

Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.