Using the Chromatographs containing the unknown mixture of Acetates and the corresponding relative response factors from the standard mixture, calculate the mole percentage of Peak 2 in the unknown mixture.
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Using the Chromatographs containing the unknown mixture of Acetates and the corresponding relative response factors from the standard mixture, calculate the mole percentage of Peak 2 in the unknown mixture.
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- 1a) Hexane, CH3(CH2)4CH3, is found to have a long retention time when separated from a mixture. A column with a C18 (18-carbon chain) stationary phase and a methanol-water modal phase is used for the separation. Explain why hexane has a long retention time. 1b)You wish to chromatographically seperate a mixtureof the follwing compunds. Which of the two compounds will be most difficult to seperatate from one another? Why?3c. A solution containing 2.50 mM X (analyte) and 1.20 mM S (standard) gave peak areas of 2509 and 7132 respectively, in a chromatographic analysis. Then, 1.00 mL of 6.00 mM S was added to 5.00 mL of unknown X and the mixture was diluted to 10.0 mL. This solution gave peak areas of 4016 and 3138 for X and S, respectively. Calculate i. the response factor for the analyte ii. the concentration of S (mM) in the 10.0 mL of mixed solutioniii. the concentration of X (mM) in the 10.0 mL of mixed solutioniv. the concentration of X in the original unknown solutionPlease answer Questions 5-3, 5-4, and 5-5 (GC Gas Chromatography) 5-2. A sample mixture of ethyl benzoate (bp 212 Celsius) and dodecane (bp 216.2 Celsius) is injected on two GC columns.Column A has DC710 silicone oil as the stationary phase, and column B uses polyethylene glycol as the stationaryphase. Which substance would be certain to elute first from column A and would the same material be expected toelute first from column B? Which column, A or B, would be expected to give the better separation of these two substances? 5-3. Question 5-2 refers to separating a mixture of two high boiling liquids by gas chromatography. These materials havesimilar boiling points. List several GC variables and conditions that would make it easier to separate thesesubstances by gas chromatography. 5-4. Capillary GC columns have better resolution than packed columns even though the enormous surface area provided by the packing material is absent in capillary columns. Why? 5-5. Preparative GC requires…
- 110.89 mg of an impure sample of the drug was diluted in 100 ml and injected in a HPLC, the AUC of the major peak in the chromatogram integrated 1294 units. A standard solution containing 1.01 mg/ml of this drug was then injected in the same HPLC and the resulting peak integrated 2228 units. What is the %w/w of purity for this sample?(One decimal place, no units)A mixture of three components is separated using paper chromatography, and each component is analyzed using absorption. With standards containing 100 ppm of each component run simultaneously, the following data were obtained: cmpd A, 0.72 absorbance; B, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identical conditions were as follows: cmpd A, 0.15 absorbance, B, 0.84; C, 0.75. a) Calculate the relative percentage composition of the unknown mixture. b) The distances traveled by the three components were 8.5, 6.3, and 5.5 cm, respectively. Sketch the appearance of the paper chromatogram. Label each spot. c) Calculate the Rf values if the solvent front is 18 cm.A standard solution containing 6.3 x 10-8 M iodoacetone and 2.0 x 10-7 M p-dichlorobenzene (an internal standard) gave peak areas of 395 and 787, respectively, in a gas chromatogram. A 3 mL unknown solution of iodoacetone was treated with 0.100 mL of 1.6 x 10-5 M p-dichlorobenzene and the mixture was diluted to 10 mL. Gas chromatography gave peak areas of 633 and 520 for iodoacetone and p-dichlorobenzene respectively. Find the concentration of the iodoacetone in the 3 mL of original unknown.
- Gas chromatography cannot analyze mixtures due to the sensitivity of the instrument. True FalseWhich solvent mixture is best for TLC chromatography of syn- and azobenzene: 75:25 DCM: Hexanes 25:75 Hexane: Acetone 90:20 Hexanes: EthanolA spectrophotometric method for the quantitative determination of the concentration ofPb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6 mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL, yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of blood.
- Oral rehydration salts are stated to contain the following components: Sodium Chloride 3.5g Potassium Chloride 1.5g Sodium Citrate 2.9g Anhydrous Glucose 20.0g 8.342 g of oral rehydration salts are dissolved in 500 ml of water. 5 ml of the solution is diluted to 100 ml and then 5 ml is taken from the diluted sample and is diluted to 100 ml. The sodium content of the sample is then determined by flame photometry. The sodium salts used to prepare the mixture were: Trisodium citrate hydrate (C6H5Na3O7, 2H2O) MW 294.1 and sodium chloride (NaCl) NW 58.5. Atomic weight of Na = 23. The content of Na in the diluted sample was determined to be 0.3210 mg/100 ml. Determine the % of stated content of Na in the sample. The stated should be 104.5, how??Oral rehydration salts are stated to contain the following components: Sodium Chloride 3.5g Potassium Chloride 1.5g Sodium Citrate 2.9g Anhydrous Glucose 20.0g 8.342 g of oral rehydration salts are dissolved in 500 ml of water. 5 ml of the solution is diluted to 100 ml and then 5 ml is taken from the diluted sample and is diluted to 100 ml. The sodium content of the sample is then determined by flame photometry. The sodium salts used to prepare the mixture were: Trisodium citrate hydrate (C6H5Na3O7, 2H2O) MW 294.1 and sodium chloride (NaCl) NW 58.5. Atomic weight of Na = 23. The content of Na in the diluted sample was determined to be 0.3210 mg/100 ml. Determine the % of stated content of Na in the sample.One microliter of 0.20 μg/mL0.20 μg/mL benzene is injected into a gas chromatograph using splitless injection. How much benzene is loaded onto the column? How much benzene is loaded onto the column if a split injection with a split ratio of 100:1100:1 is used? If both peak widths are 1 s, what is the mass of carbon entering the detector for splitless and split injection?