7. What will be the appearance of a TLC plate if a solvent of too low polarity is used for the development? A solvent oftoo high polarity Calculate the Rf value from the information given for both the known and unknown solutions and identify eachunknown solution based on the Rf values from the known solutions.distance the spottraveledRf =%3Ddistance the solvent traveledDistance the solvent travels: 6.1cmKnown SolutionsAcetaminophen in Ethanol: traveled 3.4cm2% Ibuprofen in Ethanol: traveled 5.2cm1% Caffeine in Ethanol: traveled 1.1 cm2% Aspirin in Ethanol: traveled 3.5 cmUnknown SolutionsUnknown 1: traveled 3.2 cmUnknown 2: traveled 3.7 cmUnknown 3: traveled 1.0 cmUnknown 4: traveled 4.9 cmQuestions:In Thin-Layer Chromatography, what is the stationary phase? What is the mobile phase?2. This diagram from the chemguide page shows the results of a thin layer chromatography experiment.A. Describe briefly, but precisely what would have been done to get to this stage. You can assume that you haveheight reached byhe solvent the"salvent tonbeen given a suitable thins layer chromatography plate.В.Why is the cover on the beaker?In order to identify the things in a chromatogram, you can measure the Rf value for each spot, How wouldyou work out the Rf value for each spot?С.When do you use a TLC plate coated in Silicon dioxide (SiO2)? Aluminum Oxide (Al2O3)?What error is introduced into the determination of a R, value if the top is left off the developing chamber?What will be the result of applying too much compound to a TLC plate?6. Why is it necessary to run TLC in a closed container and to have the interior vapor saturated with the solvent?3.1456

In TLC, stationery phase is used is polar in nature. components or analyte that has to seperate, has…

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Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter20: Molecular Mass Spectrometry

Section: Chapter Questions

Problem 20.11QAP

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What will be the appearance of a TLC plate if a solvent of too low polarity is used for the development? A solvent of too high polarity 7.