When you run a sequencing reaction, you should include: a) only dideoxynucleoside triphosphates (ddNTPs) . b) only deoxynucleoside triphosphates (dNTPs). c) equal amounts of dideoxynucleoside triphosphates (ddNTPs) and deoxynucleoside triphosphates (dNTPs).   d) a higher concentration of deoxynucleoside triphosphates (dNTPs) than dideoxynucleoside triphosphates (ddNTPs).   e) a higher concentration of dideoxynucleoside triphosphates (ddNTPs) than deoxynucleoside triphosphates (dNTPs).

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question
100%

When you run a sequencing reaction, you should include:

a) only dideoxynucleoside triphosphates (ddNTPs) .

b) only deoxynucleoside triphosphates (dNTPs).

c) equal amounts of dideoxynucleoside triphosphates (ddNTPs) and deoxynucleoside triphosphates (dNTPs).
 
d) a higher concentration of deoxynucleoside triphosphates (dNTPs) than dideoxynucleoside triphosphates (ddNTPs).
 
e) a higher concentration of dideoxynucleoside triphosphates (ddNTPs) than deoxynucleoside triphosphates (dNTPs).
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON