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Which is the best method for producing phase-separated GUVs (at least 20 um in size)?

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter19: Film And Screens Imaging And Processing

Section: Chapter Questions

Problem 13RQ

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Question

Which is the best method for producing phase-separated GUVs (at least 20 um in size)?

Expert Solution

Step 1

Giant unilamellar vesicles (GUVs) are used as model membrane systems whose sizes are very similar to cells

GUVs are used to study the function of highly complex bio-membranes with heterogeneous lipid composition, structure, mechanical and chemical properties.

To prepare GUVs with a complex lipid mixture one needs to mix in two heterogeneous populations of small unilamellar vesicles

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