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Examine figure a, b (shown here). If you performed the quadrant
streak plate method using a broth culture that had 10× fewer cells
than the broth used in the culture shown, which quadrant might you
expect to yield isolated colonies? Explain your answer.
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- Horizontal sequence :RIVL Vertical sequence:FMK Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below. SW algorithm. 1. Complete the scoring matrix. Scoring matrix with PAM250 scores: R I V L F M K 2. Set up, initialize and complete the SW matrix. 3. Retrace, align and score alignment(s). Use the arrows and circles for the matrix and path(s). R I V L F M K Align and score all optimal alignments here. PLZ the arrows and circles for the matrix and path(s) AND SHOW ALL possible Alignment Here the following points…Horizontal sequence :VIRL Vertical sequence:MKF Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below. SW algorithm. 1. Complete the scoring matrix. Scoring matrix with PAM250 scores: V I R L M K F 2. Set up, initialize and complete the SW matrix. 3. Retrace, align and score alignment(s). Use the arrows and circles for the matrix and path(s). V I R L M K F Align and score all optimal alignments here. PLZ the arrows and circles for the matrix and path(s) AND SHOW ALL possible AlignmentHorizontal sequence :VIRL Vertical sequence:MKF Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below. SW algorithm. 1. Complete the scoring matrix. Scoring matrix with PAM250 scores: V I R L M K F 2. Set up, initialize and complete the SW matrix. 3. Retrace, align and score alignment(s). Use the arrows and circles for the matrix and path(s). V I R L M K F Align and score all optimal alignments here.
- Horizontal sequence :RIVL Vertical sequence:FMK Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below. NW algorithm. 1. Complete the scoring matrix. Scoring matrix with PAM250 scores: R I V L F M K 2. Set up, initialize and complete the NW matrix. 3. Retrace, align and score alignment(s). Use the arrows and circles for the matrix and path(s). R I V L F M K Align and score all optimal alignments here. PLZ the arrows and circles for the matrix and path(s) AND SHOW ALL possible Alignment Here the following…For each individual on each gel, determine if the individual is homozygous for presence of the Alu insertion(++), heterozygous (+-), homozygous for the absence of the Alu insertion (-), or if the results are inconclusive (I) (explain what makes you decide your determination, in detail). Explain what may have caused any unexpected bands (neither 850 nor 550) or lack of bands.Why is this technique used?
- Gel Electrophoresis is used often in forensics. Look at the following gel to the left. From the evidence DNA, which individual matches the DNA evidence left at the crime scene?For each individual on gel, determine if the individual is homozygous for presence of the Alu insertion (++), heterozygous (+-), homozygous for the absence of the Alu insertion (- -), or if the results are inconclusive (I) (explain in detail what leads to your determination of each individual on the gel and why). Explain what may have caused any unexpected bands (neither 850 nor 550) lack of bands.DNA figure