You are conducting a biochemical experiment with an enzyme that has optimal activity at pH = 10.50. You decide to use carbonate (pKa1 = 6.38, pKa2 = 10.30) as the buffer to keep the pH stable throughout the enzymatic reaction. (Recall that the formula for carbonic acid is H2CO3.) You prepare a 0.6 M solution of carbonate buffer at pH = 10.50. Calculate the concentrations of the major carbonate species

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Chapter1: Chemical Foundations
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You are conducting a biochemical experiment with an enzyme that has optimal activity at pH = 10.50. You decide to use carbonate (pKa1 = 6.38, pKa2 = 10.30) as the buffer to keep the pH stable throughout the enzymatic reaction. (Recall that the formula for carbonic acid is H2CO3.) You prepare a 0.6 M solution of carbonate buffer at pH = 10.50. Calculate the concentrations of the major carbonate species in your solution. Show your calculations.

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