Chapter 10, Problem 10Q

A1.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme Hae III.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

A2.

Summary Introduction

To calculate: The number of different fragments obtained if the human genomic DNA having 3×10⁹ nucleotide pairs per haploid genome is cleaved using the restriction enzyme EcoR I.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.

B.

Summary Introduction

To explain: The possible reason for using Hae III to cleave human DNA for human genomic libraries though they do partial digestion of DNA.

Introduction: Bacterial DNA was observed to degrade the foreign DNA present in them always. It leads to the discovery of the restriction enzymes or the restriction nucleases. Restriction nucleases are a novel class of bacterial enzymes that cleave DNA at specific sequences of nucleotides. This property was exploited by the biologists in molecular biology and biotechnology to generate DNA fragments of different sizes using different restriction enzymes with known end sequences at the site of cleavage. One to more restriction enzymes are used to obtain DNA fragments of varying length.