Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 10, Problem 1CA
Assume you have performed a
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Chapter 10 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 10 - Which two stains done in this experiment are...Ch. 10 - Do all bacteria make endospores?Ch. 10 - What is the Gram reaction of acid-fast bacteria?Ch. 10 - Using Bergeys Manual and your text, place the...Ch. 10 - Which genera listed in the previous question...Ch. 10 - Assume you have performed a Gram stain on a sample...
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- Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that step affect the end result?arrow_forwarda. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?arrow_forwardBacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed on slide and subjected to Gram staining. What do you observe under the microscope when the following steps are skipped? Please explain.a. Staining with safraninb. Staining with Gram iodinec. Decolorizationarrow_forward
- In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?arrow_forwardWhat is the function of the mordant used in the Gram staining procedure? If the iodine step is skipped, what color are Gram-negative cells observedarrow_forwardIn Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?arrow_forward
- Enumerate the advantages derived from staining bacteria. In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?arrow_forwardWhat color would gram positive cells appear if ghr safranin staining step was left out of staining procedure ? Why?arrow_forwardAn acid-staining method was performed on a bacterial smear using the dyes carboifuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following couid explain why only blue cells were present?arrow_forward
- ou make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use correct aseptic technique by not sterilizing the loop before picking up the bacterial sample for the smear, what results may be seen under the microscope?arrow_forwardIllustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 2. Gram Stainingarrow_forwardIllustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 1. Simple Stainingarrow_forward
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