EBK BIOLOGY: CONCEPTS AND APPLICATIONS
9th Edition
ISBN: 8220100477805
Author: STARR
Publisher: CENGAGE L
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Chapter 10, Problem 5CT
Summary Introduction
To determine:
The gene (A, B or C) which is responsible for a floral mutation in Arabidopsis plant and whether this mutation causes sepals and petals to form instead of stamens and carpels in the plant.
Introduction:
ABC model of floral identity demonstrates the ways by which A, B or C genes in the
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Can you answer part a-c if its true or false
a) the AP3 and PI show auto- and cross-regulatory interactions, as well as they form obligate heterodimers to carry out the B class gene function. Therefore, if there is no PI expression, AP3 expression alone is not sufficient for establishing the petal and stamen identities.
b) Angiosperm is a group of plants whose seeds are borne within a mature ovary (fruit).
c) The organ in different organisms under every variety of forms and functions due to evolutionary development from the same or a corresponding part in a common ancestor is homologous.
You conduct an experiment to study the expression of the S protein through the
tissues of your favorite plant (Arabidopsis thaliana). The morning of the experiment
you inject in the leaves a messenger RNA that codes for the synthesis of a single
protein made of two parts that are attached to each other: the functional S protein
and a red fluorescent protein tag (RFP). You perform two cross sections of the same
root, one section at the start of the experiment (time = Oh; corresponding to the time
of injection) and one section in the afternoon (time = 8h). Through fluorescent
microscopy you observe a change in the coloration inside the cells of the root's
central tissues (as indicated by the arrows): from no coloration (time = Oh) to red
(time = 8h).
• The cells of the central tissues in the roots do not have nuclei or ribosomes.
How can you explain this change of coloration? Please provide a cellular
feature that can lead to this.
• What is one advantage of using an RFP-tag in an mRNA?…
You are a developmental geneticist studying flowering time variation in Arabidopsis. You perform a
mutagenesis screen to identify mutants in the photoperiod pathway. You conduct the screen and
find two different plants that show the same mutant phenotype. You then use a complementation
test. What is the predicted outcome of this test if both phenotypes are caused by mutations in
separate genes?
recover the wild type phenotype
overexpress the gene
O recover the mutant phenotype
Chapter 10 Solutions
EBK BIOLOGY: CONCEPTS AND APPLICATIONS
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- The photos below show flowers from two Arabidopsis plants. One plant is wild-type unmutated; the other carries a mutation in one of its ABC floral identity genes. This mutation causes sepals and petals to form instead of stamens and carpels. Refer to Figure 10.7 to decide which gene A, B, or C has been inactivated by the mutation.arrow_forwardIn roses, the synthesis of red pigment is produced by two steps in a pathway. gene O magenta intermediate - gene P colorless intermediate- red pigment What would the phenotype be of a plant homozygous for a null mutation of gene P? What would the phenotype be of a plant homozygous for a null mutation of gene Q? What would the phenotype be of a plant homozygous for null mutations of genes P and Q? magenta red Match a genotype to each strain. colorless Strain P locus Q locus homozygous null mutation of gene P homozygous null mutation of gene Q homozygous null mutations of genes P and Q Answer Bank plp PIP What F2 ratio is expected from crossing a plant that is homozygous for a null mutation of gene P with a plant that is homozygous for a null mutation of gene Q? Assume independent assortment. 9 colorless : 4 magenta : 3 red 9 red : 4 colorless : 3 magenta O 9 red : 4 magenta : 3 colorlessarrow_forwardIn Figure 6-1,a. what do the yellow stars represent?b. explain in your own words why the heterozygote isfunctionally wild typearrow_forward
- You are studying a plant with tissue comprising both green and white sectors. You wish to decide whether this phenomenon is due (1) to a chloroplast mutation of the type considered in this chapter or (2) to a dominant nuclear mutation that inhibits chlorophyll production and is present only in certain tissue layers of the plant as a mosaic. Outline the experimental approach that you would use to resolve this problem.arrow_forwardYou are a developmental geneticist studying flowering time variation in Arabidopsis. You perform a mutagenesis screen to identify mutants in the photoperiod pathway. Given what you know about photoperiodism in Arabidopsis, what phenotype are you looking for and under what photoperiodic conditions would you perform the experiment? delayed flowering in long days delayed flowering in short days same flowering in short days early flowering in short days same flowering in long days early flowering in long daysarrow_forwardAnthocyanin is a pigment that gives flowers and leaves purple colors. The M gene codes for a transcription factor (Myb) that promotes expression of an enzyme that produces anthocyanin. The W gene codes for a different enzyme (Chs) that allows anthocyanin to be deposited in plant leaves and flowers. The dominant phenotype is the production of functional Myb and Chs. 1) Plants that have the mm genotype do not show any purple color. What is the best explanation for why this is? a) Anthocyanin cannot be deposited into the flowers and leaves b) Anthocyanin is not produced in the plant cells c) The M gene is codominant to the W gene d) The M gene is epistatic to the W gene 2)Assume a plant has the genotype MMww. Would this plant have any purple flowers? a) Yes b) Noarrow_forward
- Three haploid fungal mutants that require compound W for growth were isolated. Each mutant contains a recessive allele in a single gene. Three compounds (A, B and C) in the biosynthetic pathway to W are known, but their order in the pathway is unknown. Each compound is tested for its ability to support the growth of each of the three mutants. Phenotypes of all of the three mutants are shown in the following table (“+" indicates growth, "-" indicates no growth). A C W Mutant 1 Mutant 2 Mutant 3 What would be the phenotype of a haploid mutant that contains both mutant alleles in mutant 2 and 3? Phenotype refers to growth or absence of growth on compounds A, B, C and WN. O Like mutant 1 O Like mutant 2 Like mutant 3 O Like wild typearrow_forwardWhich gene encodes the enzyme at the upper boxed step in the pathway? Which gene encodes the enzyme at the upper boxed step in the pathway? gene 1 gene 2 gene 3 gene 4arrow_forwardIn Figure 6-2, explain how the mutant polypeptide actsas a spoiler and what its net effect on phenotype is.arrow_forward
- For a haploid fungus, the starting point in the biosynthesis of the amino acid arginine is Compound X, which is always present in and absorbed from the environment. The arginine biosynthetic pathway is: Enzyme A Enzyme B Enzyme Ç Compound X It is know that genes encoding enzymes A and C are on two different chromosomes. Compound Y Compound Z- Arginine A mutant strain of genotype a (lacking only enzyme A) is crossed to a mutant strain of genotype c (lacking only enzyme C) to generate a diploid strain. Sporulation (i.e. meiosis) is subsequently induced in the resulting diploid strain. What proportion of the spores (haploids formed by sporulation) is expected to grow on medium without arginine but supplemented with Compound Y? O 100% 50% 0% 25%arrow_forwardplease explain if it would be elongated or shortened expression! I know double mutant epistasis is always downstream> upstream but if upstream gene controls downstream, what happens?arrow_forwardTrace the genetic steps in the development of a peanut plant that isresistant to insect larvae (table given). (Hint: You need to useAgrobacterium.)arrow_forward
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