Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Question
Chapter 11, Problem 3AQ
Summary Introduction
To discuss:
Several genetic systems use the β-galactosidase as a reporter. The lacZ gene encodes β-galactosidase. The major advantages and disadvantages of using the luciferase or green fluorescent protein as a reporter instead of the β-galactosidase.
Concept introduction:
The lacZ gene from E. coli encodes β-galactosidase enzyme, which is required for catabolism of lactose. The cells are plated on X-gal (5-bromo4-chloro-3-indolyl-b-D-galactopyranoside) containing media. Cells expressing β-galactosidase enzyme can be analyzed by their color of the colonies. X-gal, the chemical substance is cleaved by β-galactosidase enzyme and produces blue color colonies.
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Chapter 11 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 11.1 - Prob. 1MQCh. 11.1 - Prob. 2MQCh. 11.2 - Prob. 1MQCh. 11.2 - Prob. 2MQCh. 11.3 - Why is a primer needed at each end of the DNA...Ch. 11.3 - MINIQUIZ
• From which organisms are thermostable...Ch. 11.3 - How does RT-PCR differ from traditional PCR?Ch. 11.4 - What is the purpose of molecular cloning?Ch. 11.4 - Prob. 2MQCh. 11.4 - Prob. 3MQ
Ch. 11.5 - Prob. 1MQCh. 11.5 - How can site-directed mutagenesis be useful to...Ch. 11.5 - What are knockout mutations?Ch. 11.6 - Prob. 1MQCh. 11.6 - Prob. 2MQCh. 11.7 - Prob. 1MQCh. 11.7 - Prob. 2MQCh. 11.7 - Prob. 3MQCh. 11.8 - Prob. 1MQCh. 11.8 - Prob. 2MQCh. 11.9 - MINIQUIZ
• Describe the components needed for an...Ch. 11.9 - Prob. 2MQCh. 11.10 - Prob. 1MQCh. 11.10 - Prob. 2MQCh. 11.10 - Prob. 3MQCh. 11.11 - What major advantage does cloning mammalian genes...Ch. 11.11 - Prob. 2MQCh. 11.12 - Prob. 1MQCh. 11.12 - Prob. 2MQCh. 11.12 - Prob. 3MQCh. 11.13 - Prob. 1MQCh. 11.13 - Give an example of a genetically modified plant...Ch. 11.13 - How have transgenic salmon been engineered to...Ch. 11.14 - Explain why recombinant vaccines might be safer...Ch. 11.14 - Prob. 2MQCh. 11.15 - Explain why metagenomic cloning gives large...Ch. 11.15 - Prob. 2MQCh. 11.16 - Prob. 1MQCh. 11.16 - Prob. 2MQCh. 11.17 - What are biobricks?Ch. 11.17 - How was Escherichia coli modified to produce a...Ch. 11 - Prob. 1RQCh. 11 - Prob. 2RQCh. 11 - Describe the basic principles of gene...Ch. 11 - Prob. 4RQCh. 11 - Prob. 5RQCh. 11 - Prob. 6RQCh. 11 - Prob. 7RQCh. 11 - REVIEW QUESTIONS
8. What is a reporter gene?...Ch. 11 - Prob. 9RQCh. 11 - Prob. 10RQCh. 11 - Prob. 11RQCh. 11 - Prob. 12RQCh. 11 - Prob. 13RQCh. 11 - Prob. 14RQCh. 11 - Prob. 15RQCh. 11 - Prob. 16RQCh. 11 - Prob. 17RQCh. 11 - What is the Ti plasmid and how has it been of use...Ch. 11 - What is a subunit vaccine and why are subunit...Ch. 11 - How has metagenomics been used to find novel...Ch. 11 - Prob. 21RQCh. 11 - Prob. 22RQCh. 11 - Prob. 1AQCh. 11 - Suppose you have just determined the DNA base...Ch. 11 - Prob. 3AQCh. 11 - Prob. 4AQ
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