CUSTOM PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781266032844
Author: WILLEY
Publisher: MCG
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Chapter 11.8, Problem 4CC
Summary Introduction
The fermentation process yields less ATP when compared to the aerobic respiration. Since the fermentation, process lacks an electron transport chain to carry the terminal electron to generate proton motive force. The fermentation is considered to be a
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How many net molecules of ATP and NADH are produced in "anaerobic glycolysis" (glycolysis + fermentation) starting from 1 molecule of fructose 1,6-bisphosphate and ending with 2 molecules of lactate? (Reminder: you are starting with one molecule of fructose 1,6-bisphosphate in this problem! Not from glucose!)
Draw OUT THE arrow pushing reaction mechanism for the following steps of glycolysis:
SHOW/IDENTIFY the arrow pushing mechanism in at least 2 steps.
CH,OPO
CH,OH
hexokinase
2+
H
H
H
H
H
H
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+ ATP
OH
H
ADP + H+
H
ОН
+
НО
HO
OH
H
ОН
H
ОН
Glucose-6-phosphate
(G6P)
Glucose
How many ATP or NADH molecules are produced or required in each of the following steps in glycolysis?
Match the words in the left column to the appropriate blanks in the sentences on the right.
zero
one
two
produced
required
In the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate,
NADH molecules are
In the conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate,
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In the conversion of phosphoenolpyruvate to pyruvate
molecules are
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NADH
Chapter 11 Solutions
CUSTOM PRESCOTT'S MICROBIOLOGY
Ch. 11.1 - Prob. 1CCCh. 11.1 - Retrieve, Infer, Apply Compare...Ch. 11.1 - Retrieve, Infer, Apply What are the three major...Ch. 11.2 - Is NAD+ reduced to NADH in the catabolic or...Ch. 11.2 - Prob. 1CCCh. 11.2 - Why is it to a cells advantage to catabolize...Ch. 11.2 - Prob. 3CCCh. 11.4 - Which reactions are examples of substrate-level...Ch. 11.4 - For what kinds of reactions is NADPH used?Ch. 11.4 - For what macromolecule is ribose 5-phosphate a...
Ch. 11.4 - Summarize the major features of the...Ch. 11.4 - Prob. 2CCCh. 11.5 - Identify the substrate and products of the TCA...Ch. 11.5 - What chemical intermediate links pyruvate to the...Ch. 11.5 - Prob. 3CCCh. 11.5 - Retrieve, Infer, Apply In what eukaryotic...Ch. 11.5 - Why is it desirable for a microbe with the...Ch. 11.6 - Prob. 1MICh. 11.6 - Prob. 2MICh. 11.6 - Describe the current model of oxidative...Ch. 11.6 - Prob. 3CCCh. 11.6 - Prob. 4CCCh. 11.7 - Prob. 1CCCh. 11.7 - Prob. 2CCCh. 11.7 - Prob. 3CCCh. 11.8 - Prob. 1MICh. 11.8 - Prob. 1CCCh. 11.8 - Prob. 2CCCh. 11.8 - Prob. 3CCCh. 11.8 - Prob. 4CCCh. 11.8 - Prob. 5CCCh. 11.9 - What is the difference between a hydrolase and...Ch. 11.9 - Prob. 2MICh. 11.9 - Retrieve, Infer, Apply Briefly discuss the ways in...Ch. 11.9 - Prob. 2CCCh. 11.9 - Retrieve, Infer, Apply Describe how a...Ch. 11.11 - How do chemolithotrophs obtain their ATP and...Ch. 11.11 - Prob. 2CCCh. 11.11 - Why can hydrogen-oxidizing bacteria and archaea...Ch. 11.11 - What is reverse electron flow and why do many...Ch. 11.11 - Prob. 5CCCh. 11.12 - When electrons from P700 are used to reduce NADP+,...Ch. 11.12 - Define the following terms: light reactions, dark...Ch. 11.12 - Prob. 2CCCh. 11.12 - What is the function of accessory pigments?Ch. 11.12 - Prob. 4CCCh. 11.12 - Compare and contrast anoxygenic phototrophy and...Ch. 11.12 - Prob. 6CCCh. 11 - Prob. 1RCCh. 11 - Prob. 2RCCh. 11 - Prob. 3RCCh. 11 - Prob. 4RCCh. 11 - Prob. 5RCCh. 11 - Prob. 6RCCh. 11 - How would you isolate a thermophilic...Ch. 11 - Certain chemicals block ATP synthesis by allowing...Ch. 11 - Prob. 3ALCh. 11 - Review the description of the Berkeley Pit Lake in...Ch. 11 - Prob. 5AL
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- ATP synthase, hexokinase, and isocitrate dehydrogenase are important enzymes in aerobic respiration. Please provide a description for each enzyme containing the following information: (1) the metabolic stage in aerobic respiration they are involved in, (2) the enzyme class where they belong, and (3) the chemical eaction they catalyze.arrow_forwardHow many ATPs could theoretically be formed from a glucosemolecule carried through aerobic respiration, and how many areprobably formed?arrow_forwardWhat steps in glycolysis generate substrate-level phosphorylation, and how many ATP molecules are generated in this way in this pathway? How does the generation of ATP by substrate-level phosphorylation differ from ATP generation by oxidative phosphorylation?arrow_forward
- The condensation reaction catalyzed by ß-ketoacyl-ACP synthase synthesizes a four-carbon unit by combining a two-carbon unit and a three-carbon unit, with the release of CO₂. What is the thermodynamic advantage of this process over one that simply combines two two-carbon units? The reaction is reversible and does not require the input of ATP. The exergonic release of CO₂ drives the irreversible reaction in the direction of fatty acid synthesis. The release of CO₂ is endergonic and results in the production of ATP. The reaction is endergonic and requires the input of ATP.arrow_forwardConsider the steps of the krebs cycle and electron carrier chain and answer: a) How many ATPs are produced from 10 Pyruvato molecules? b) How many ATPs are produced from 12 Acetyl CoA molecules?arrow_forwardName the three main catabolic pathways and the estimated ATP yield for each.arrow_forward
- The following reactions are encountered in the metabolic cycles. Give the main class of enzymes that catalyzes these reactions. glucose + pyruvate → α-ketoglutarate + alanine lactate + NAD+ → pyruvate + NADH + H+ D-ribulose-5-phosphate → D-xylulose-5-phosphate fructose-1-phosphate → fructose-6-phosphate glucose + ATP → glucose-6-phosphate + ADP starch + HOH → α-D-glucose acetyl CoA + oxaloacetate → citric acid pyruvate + CO2 + ATP → oxaloacetate + ADP + Pi creatine + ATP → phosphocreatine + ADP alanine → propanoic acid + NH3 ala-gly-val → ala + gly + val acetylcholine ester → choline + acetic acid triglyceride → 3 fatty acids + glycerol deoxyribose + bases (A, G, C, T) + Pi → DNA glyceraldehyde-3-phosphate ↔ dihydroxyacetone phosphatearrow_forwardThe following are reactions encuntered in the metabolic cycles. Give the main class of enzymes that catalyzes these reactions 1. Alanine + Oxoglutarate ---> Pyruvate +Glutamatearrow_forwardWhat is the overall net reaction of glycolysis? C6H12O6 + 2 NADH + 2 P₁ + 2 ATP --> 2 C3H3O3 + 2 NAD+ + 2 H+ + 2 H₂O + 2 ADP C6H12O6 + 2 NAD+ + 4 P₁ + 4 ADP --> 2 C3H3O3 + 2 NADH + 2 H+ + 2 H₂O + 4 ATP Glucose + 2 NAD+ + 2 P₁ + 2 ADP --> 2 C3H3O3 + 2 NADH + 2 H+ + 2 H₂O + 2 ATP Glucose + 2 FAD + 2 P; + 2 ADP --> 2 Pyruvate + 2 FADH₂ + 2 H+ + 2 H₂O + 2 ATP C6H12O6 + 2 NAD+ + 2 P₁ + 2 ADP --> Pyruvate + 2 NADH + 2 H+ + 2 H₂O + 2 ATParrow_forward
- The overall reactions for gluconeogenesis and glycolysis are given below. What is the energy cost (in ATP equivalents) of transforming one molecule of glucose to pyruvate (via glycolysis) and back to glucose (via gluconeogenesis)? Gluconeogenesis: 2 pyruvate + 4 ATP + 2 GTP + 2NADH + 4H₂O Glycolysis: Glucose + 2ADP + 2P₁ + 2NAD+ - 2 pyruvate + 2ATP + 2NADH + 2H+ + 2H₂000 Select one: O a. 2 ATP O b. 4 ATP O c. 6 ATP O d. 8 ATP Prev Page glucose + 4ADP + 2GDP + 2P₁ + 2NAD+ + 2H+ MI 21 22 00:508D DAD 1=1 SE 30: DONDOK 23 24 25 26 27 28 29 30 Report question issue B Next Page Garrow_forwardThe glucose/glucose-6-phosphate substrate cycle involves distinct reactions of glycolysis and gluconcogenesis that interconvert these two metabolites. Assume that under physiological conditions, [ATP] = [ADP] and [Pi] =1 mM. Consider the following glycolytic reaction catalyzed by hexokinase: ATP + glucose = AG' = -16.7 kJ/mol ADP + glucose-6-phosphate (a) Calculate the equilibrium constant (K) for this reaction at 298 K, and from that, calculate the maximum [glucose-6-phosphate]/[glucose] ratio that would exist under conditions where the reaction is still thermody- namically favorable. (b) The reverse of this interconversion in gluconeogenesis is catalyzed by glucose-6-phosphatase: glucose-6-phosphate + H,0 = glucose + P, AGr = -13.8 kJ/mol K= 262 for this reaction. Calculate the maximum ratio of [glucose]/ [glucose-6-phosphate] that would exist under conditions where the reaction is still thermodynamically favorable. (c) Under what cellular conditions would both directions in the…arrow_forwardWhat compounds with high phosphate group-transfer potential are synthesized during glycolysis?arrow_forward
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