EBK CAMPBELL BIOLOGY:CONCEPTS+CONNECT.
9th Edition
ISBN: 9780134536231
Author: Taylor
Publisher: PEARSON CO
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Chapter 12, Problem 10TYK
A biologist isolated a gene from a human cell, inserted it into a plasmid, and inserted the plasmid into a bacterium. The bacterium made a new protein, but it was nothing like the protein normally produced in a human cell. Why? (Explain your answer.)
a. The bacterium had undergone transformation.
b. The gene did not have sticky ends.
c. The human gene contained introns.
d. The gene was not synthesized from scratch.
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Students have asked these similar questions
What is a cloning vector?
A.
The DNA probe used to locate a particular gene in the genome.
B.
An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell.
C.
The laboratory apparatus used to clone genes.
D.
An enzyme that cuts DNA into restriction fragments.
A. What is the pathogen that is attacking bananas today?b. Why is this especially problematic in Africa?
C. Why do we expect to lose the war with this pathogen?d. What is random mutagenesis?
Arrange the following steps in the sequence they would happen in a DNA cloning experiment.
a. sealing DNA fragments into vectors with DNA ligase;
b. utilizing a probe to detect a clone in the library;
c. sequencing the clone's DNA;
d. creating a DNA library of clones;
e. cutting genomic DNA with restriction enzymes.
A.
e,a,d,b,c
B.
a,d,b,c,e
C.
c,b,e,a,d
D.
e,d,a,c,b
Chapter 12 Solutions
EBK CAMPBELL BIOLOGY:CONCEPTS+CONNECT.
Ch. 12 - Imagine you have found a small quantity of DNA....Ch. 12 - Which of the following would be considered a...Ch. 12 - The DNA profiles used as evidence in a murder...Ch. 12 - A paleontologist has recovered a tiny bit of...Ch. 12 - How many genes are there in a human sperm cell? a....Ch. 12 - When a typical restriction enzyme cuts a DNA...Ch. 12 - Why does DNA profiling rely on comparing specific...Ch. 12 - Recombinant DNA techniques are used to...Ch. 12 - A biochemist hopes to find a gene in human cells...Ch. 12 - A biologist isolated a gene from a human cell,...
Ch. 12 - Explain how you might engineer E. coli to produce...Ch. 12 - What is left for genetic researchers to do now...Ch. 12 - Today, it is fairly easy to make transgenic plants...Ch. 12 - In the not-too-distant future, gene therapy may be...Ch. 12 - The possibility of extensive genetic testing...Ch. 12 - SCIENTIFIC THINKING Scientists investigate...
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- Why is DNA ligase so important in recombinant DNA technology? a.) It causes DNA to make multiple copies of itself. b.) It joins two DNA fragments together. c.) It shapes bacterial DNA into a circular plasmid. d.) It cuts DNA into restriction fragments.arrow_forwardA contig is a. a set of molecular markers used in gene mapping. b. a set of overlapping fragments that form a continuous stretch of DNA. c. a set of fragments generated by a restriction enzyme. d. a small DNA fragment used in sequencing.arrow_forwardFrom where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistryarrow_forward
- a) what are restriction enzymes? b) What is the main function of restriction enzymes in nature? c) Compare and contrast the these enzymes in nature and in scientific research.arrow_forwardIn genetic engineering, can we insert the gene from human to E. coli (bacteria)? Explain your answer.arrow_forwardWould it be possible to use human polymerase for the PCR reaction? a. No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur. b. No, because human polymerase cannot be extracted from cells to use in a lab setting. c. Yes, because we are using human DNA as the template DNA. d. Yes, because human polymerase can add bases to a template strand without a primer.arrow_forward
- If you knew the sequence of a gene in one organism, how could you determine if another organism had a similar gene? A. insert the known gene into a vector and use the vector to insert the known gene into the other organism B. treat the genomes of both organisms with the same restriction enzyme and compare the patterns of the bands produced with gel electrophoresis C. create a hybrid of the two organisms by breeding them and check for mutations D. create labeled DNA probes from the known gene and use them to search the genome of the other organismarrow_forwardWhat is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbonesarrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forward
- What is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forwardExplain how recombinant DNA technology uses restriction enzymes (restriction endonucleases). Explain how recombinant DNA technology uses DNA ligase enzymes.arrow_forwardIn next-generation sequencing, which of these advances allows for massively parallel sequencing? a. Pieces of DNA are fixed to a surface, so we can tell which new nucleotides were added to each piece. b. DNA sequences are read in real-time as nucleotides are added to each piece. c. Each segment of the genome can be pieced back together through shotgun alignment d. Single molecules of DNA can be read without the need for amplification.arrow_forward
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