Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 12, Problem 17P
A
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A serial dilution of a bacterial culture yields the following number of colonies. Which plate(s) should be used to determine the original cell density?
Plate A
Plate B
Plate C
Plate D
Plate E
Dilution Factor
10-5
10-6
10-7
10-8
10-9
# of Colonies
Too many to count
850
456
80
14
Group of answer choices
All of these choices
A & B
D & E
E
None of these choices
A
B & C
D
B
C & D
C
You were asked to prepare a dilution series of a bacterial culture where only 3 tubes will be used (all with 5 mL total solution). Draw a schematic diagram to make tube 1 have a 10-2 dilution, tube 2 have 10-3 dilution, and tube 3 to have 10-5 dilution
You were instructed to dilute an antibiotic solution 1/10, redilute 1/25, and again 1/50, then you need to make 100mL of each dilution. How would you go about preparing this dilution series? (Present your answer in a schematic diagram)
What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2?
What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?
Chapter 12 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 12 - 11.1 Identify two general ways chemical mutagens...Ch. 12 - 11.2 Nitrous acid and (BU) alter DNA by different...Ch. 12 - 11.19 Using the adeninethymine base pair in this...Ch. 12 - The partial amino acid sequence of a wild-type...Ch. 12 - 5. Thymine is usually in its normal, common form....Ch. 12 - Ultraviolet (UV) radiation is mutagenic.
What...Ch. 12 - Researchers interested in studying mutation and...Ch. 12 - The effect of base - pair substitution mutations...Ch. 12 - Prob. 9PCh. 12 - 11.10 In numerous population studies of...
Ch. 12 - 11.11 Two different mutations are identified in a...Ch. 12 - 11.22 Many human genes are known to have homologs...Ch. 12 - 11.13 Answer the following questions concerning...Ch. 12 - Prob. 14PCh. 12 - Prob. 15PCh. 12 - Prob. 16PCh. 12 - 11.15 A sample of the bacterium is exposed to...Ch. 12 - 11.16 A strain of is identified as having a null...Ch. 12 - Define gene conversion and contrast it with gene...Ch. 12 - Prob. 20PCh. 12 - Prob. 21PCh. 12 - Prob. 22PCh. 12 - Prob. 23PCh. 12 - Prob. 24PCh. 12 - 25. If homologous recombination did not occur,...Ch. 12 - In this chapter, three features of genes or of DNA...Ch. 12 - Briefly compare the production of DNA double -...Ch. 12 - During mismatch repair, why is it necessary to...Ch. 12 - 11.27 Following the spill of a mixture of...Ch. 12 - A geneticist searching for mutations uses the...Ch. 12 - A wild - type culture of haploid yeast is exposed...Ch. 12 - A fragment of a wild - type polypeptide is...Ch. 12 - Prob. 33PCh. 12 - 11.40 Common baker’s yeast () is normally grown at...Ch. 12 - 11.41 The two gels illustrated below contain...Ch. 12 - Alkaptonuria is a human autosomal recessive...Ch. 12 - 11.33 In an experiment employing the methods of...Ch. 12 - Using your knowledge of DNA repair pathways choose...Ch. 12 - 11.35 Ataxia telangiectasia is a human inherited...Ch. 12 - 40. Two haploid strains of fungus are fused to...
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