EBK CAMPBELL BIOLOGY:CONCEPTS+CONNECT.
8th Edition
ISBN: 9780133890310
Author: Reece
Publisher: PEARSON CO
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Textbook Question
Chapter 12, Problem 4TYK
A paleontologist has recovered a tiny bit of organic material from the 400-year-old preserved skin of an extinct dodo. She would like to compare DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of DNA available for testing?
a. restriction fragment analysis
b. polymerase chain reaction
c. molecular probe analysis
d. electrophoresis
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The following question is related to Restriction Enzymes and RFLP.
Using EcoRl, how many cuts were performed on DNA sequence A?
A.) 2
B.) 3
C.) 4
The following question is related to Restriction Enzymes and RFLP.
Using EcoRl, how many fragments were PRODUCED in DNA sequence A?
A.) 2
B.) 3
C.) 4
Would it be possible to use human polymerase for the PCR reaction?
a.
No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur.
b.
No, because human polymerase cannot be extracted from cells to use in a lab setting.
c.
Yes, because we are using human DNA as the template DNA.
d.
Yes, because human polymerase can add bases to a template strand without a primer.
Chapter 12 Solutions
EBK CAMPBELL BIOLOGY:CONCEPTS+CONNECT.
Ch. 12 - Imagine you have found a small quantity of DNA....Ch. 12 - Which of the following would be considered a...Ch. 12 - The DNA profiles used as evidence in a murder...Ch. 12 - A paleontologist has recovered a tiny bit of...Ch. 12 - How many genes are there in a human sperm cell? a....Ch. 12 - When a typical restriction enzyme cuts a DNA...Ch. 12 - Why does DNA profiling rely on comparing specific...Ch. 12 - Recombinant DNA techniques are used to...Ch. 12 - A biochemist hopes to find a gene in human cells...Ch. 12 - Prob. 10TYK
Ch. 12 - Explain how you might engineer E. coli to produce...Ch. 12 - What is left for genetic researchers to do now...Ch. 12 - Today, it is fairly easy to make transgenic plants...Ch. 12 - In the not-too-distant future, gene therapy may be...Ch. 12 - The possibility of extensive genetic testing...Ch. 12 - SCIENTIFIC THINKING Scientists investigate...
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- Please answer these two questions regarding PCR: a) Why do you need to perform PCR on DNA obtained from a crime scene? b) Why so forensic labs analyze non-coding DNA rather than genes?arrow_forwardWhy is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.arrow_forwardQuantitative PCR differs from regular PCR in that it uses [A] to [B] the amount of [C] in a sample. It cannot quantify [D] unless it is first made into [F]. Match each of the following to its appropriate letter: quantify, cDNA, RNA, DNA or RNA, fluorescence. 1) A 2) B 3) C 4) D 5) E Here are the choices for the questions a) quantify b) RNA c) cDNA d) fluorescence e) DNA or RNAarrow_forward
- Can you help me with this question, please? What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one personarrow_forwardChoose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)? a. PCR requires a copy of RNA that serves as a template b. Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA c. Annealing requires cooler temperatures than denaturation d. Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA e. PCR is a quick way of using minute quantities of DNA to create millions of copiesarrow_forwardIf a DNA sample was GC-rich, what would you need to do to adjust your PCR reaction and why?arrow_forward
- During DNA isolation, you incubated the tissue sample at 55°C for 60 minutes, followed by incubation at 95°C for 10 minutes. What happens to the DNA molecules in this last 95°C incubation? What would happen to the PCR if this step was omitted?arrow_forwardA DNA fingerprint is based on _____. a repeating sequences found on noncoding regions b the presence of restriction enzymes c a single repeating region d repeating sequences found on coding regionsarrow_forwardRegarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forward
- From where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistryarrow_forwardPart A. There are three temperatures used in PCR: 55 degrees C, 72 degrees C, and 95 degrees C. The denaturation step occurs at _ _ _ _ C. The annealing step occurs at _ _ _ _ C. The elongation step occurs at _ _ _ _ C. Part B. What component of the PCR allows the reaction to amplify a specific sequence? a. dNTPs b. DNAPol c. Template d. Primerarrow_forwardPCR (polymerase chain reaction) is an excellent method of generating copies of target DNA. If a single piece of double stranded DNA (dsDNA) is put into a PCR machine, how many dsDNA segments will there be after 3 rounds? A. 8 segments, with 2 original strands paired B. 16 segments, with 2 original strands on different segments C. 16 segments, with 2 original strands paired D. 8 segments, with 2 original strands on different segmentsarrow_forward
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