ESSENTIALS OF GENETICS-W/MOD.ACCESS
ESSENTIALS OF GENETICS-W/MOD.ACCESS
9th Edition
ISBN: 9780134452876
Author: KLUG
Publisher: PEARSON
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Chapter 12, Problem 8PDQ

When the amino acid sequences of insulin isolated from different organisms were determined, some differences were noted. For example, alanine was substituted for threonine, serine was substituted for glycine, and valine was substituted for isoleucine at corresponding positions in the protein. List the single-base changes that could occur in triplets to produce these amino acid changes.

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Lactose permease, a protein of E. coli, is composed of a singlepolypeptide that is 417 amino acids in length. By convention, theamino acids within a polypeptide are numbered from the aminoterminalend to the carboxyl-terminal end. Are the following questionsabout lactose permease true or false?A. Because the sixty-fourth amino acid is glycine and the sixty- eighth amino acid is aspartic acid, the codon for glycine,64, is closer to the 3′ end of the mRNA than the codon for aspartic acid, 68.B. The mRNA that encodes lactose permease must be greater than1241 nucleotides in length.
What does this important observation imply about the relation between the amino acid sequence of insulin and its three-dimensional structure?
What is the molecular weight of the Botulinum neurotoxin, a protein that contains 1350 amino acids? Assume an average distribution of amino acids

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