MASTERING BIOLOGY 9E
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ISBN: 9780136560623
Author: Campbell
Publisher: PEARSON
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Textbook Question
Chapter 12, Problem 8TYK
Recombinant DNA techniques are used to custom-build bacteria for two main purposes: to obtain multiple copies of certain genes and to obtain useful proteins produced by certain genes. Give an example of each of these applications in medicine and agriculture.
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describe how the bacteria should have the ability to be recovered back from the infected host for the purpose of experimentation including sequencing ,vitro culturing and polymerase chain reaction amplification.
Give an example of application of recombinant DNA in the following fields: crop production, medicine, industry. Briefly discuss how recombinant DNA is applied in each.
Which of the following correctly describes a possible scenario during a run of gel electrophoresis with DNA samples?
Chapter 12 Solutions
MASTERING BIOLOGY 9E
Ch. 12 - Imagine you have found a small quantity of DNA....Ch. 12 - Which of the following would be considered a...Ch. 12 - The DNA profiles used as evidence in a murder...Ch. 12 - A paleontologist has recovered a tiny bit of...Ch. 12 - How many genes are there in a human sperm cell? a....Ch. 12 - When a typical restriction enzyme cuts a DNA...Ch. 12 - Why does DNA profiling rely on comparing specific...Ch. 12 - Recombinant DNA techniques are used to...Ch. 12 - A biochemist hopes to find a gene in human cells...Ch. 12 - A biologist isolated a gene from a human cell,...
Ch. 12 - Explain how you might engineer E. coli to produce...Ch. 12 - What is left for genetic researchers to do now...Ch. 12 - Today, it is fairly easy to make transgenic plants...Ch. 12 - In the not-too-distant future, gene therapy may be...Ch. 12 - The possibility of extensive genetic testing...Ch. 12 - SCIENTIFIC THINKING Scientists investigate...
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- What are secondary metabolites? In terms of biotechnology, how do we get bacteria to produce them with cloning technology? Explain the stages in schematics and explanations.arrow_forwardDescribe an experiment for testing to determine whether or not the bacteria have been successfully transformed.arrow_forwardIn your opinion, is antibiotic use at farm sites promote the development and spread of antibiotic resistant genes in bacteria? Why or why not please explain based on the articlearrow_forward
- In your own words, explain how bacteria can be manipulated to contain a foreign DNA sequence or gene, how the expression of the gene could be monitored or selected for in the lab, and how the protein coded from this DNA sequence could potentially be purified. You must include at least 6 of the following terms in your answer: transformation, plasmid, antibiotic selection, fusion protein, chromatography, reporter gene, bioluminescence, competent cells, electroporation, calcium chloride.arrow_forwarddescribe the blue-white colony selection method in the recombinant colony screening, and present a brief argument for its use in biotechnology labs.arrow_forwardName the five key tools for accomplishing the tasks of recombinant DNA technology.also mention the function of each tool.arrow_forward
- Discuss how recombinant DNA technology has improved the production of the following: Restriction endonucleases Indigo dye Microbial pollutant biosensors Xanthan gum Pst1 cholera vaccinearrow_forward1.1 Give an overview of the positive and negative effects of microbial biotechnology in agriculture. 1.2 Give a detailed explanation of how recombinant DNA technology can be used for the management and control of emerging diseases. In your answer, provide 5 applications and give at least 2 relevant examples for each.arrow_forwardHow can site-specific recombination be used in recombinant DNA technology? Explain with an examplearrow_forward
- True or False: Plasmids can ONLY be used in transforming bacterial cells.arrow_forwardThe presence of colonies on the bacterial plate post transformation suggests which of the following? Group of answer choices The colonies contain the transformed plasmid and are not resistant to the antibiotic The colonies contain the transformed plasmid and are resistant to the antibiotic The colonies do not contain the transformed plasmid and are not resistant to the antibiotic The colonies do not contain the transformed plasmid and are resistant to the antibioticarrow_forwardExplain how biotechnology and bioinformatics are used to identify microorganisms Explain why biotechnology has replaced many of the traditional diagnostic tools in microbiology Explain why a PCR reaction needs a source of nucleotides in the form of ATP, GTP, CTP and TTP. Explain what primers are and describe their use in PCR reactions Given the results from a PCR/Gel electrophoresis or a DNA sequence; determine the identity of a bacterium or virus...arrow_forward
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