Chapter 14, Problem 18Q

A.

Summary Introduction

To explain: What would be the effect if a purified bacteriorhodopsin and purified ATP synthase from ox-heart mitochondria together is reconstituted to the same membrane vesicles and then the ADP, P_i, and light are added to the external medium.

Concept introduction:

Bacteriorhodopsin present in archeal cells is a protein that acts as a proton pump that can pump protons across the membrane in the presence of light energy. ATP synthase is an ion pump, molecular motor, and is an enzyme that catalyzes the production of ATP. These two different proteins from the different sources are added to the vesicle, and ATP is produced in the vesicle.

B.

Summary Introduction

To explain: What would be the effect if not all the detergent are removed and the vesicle membrane therefore remains leaky to ions.

Concept introduction:

Bacteriorhodopsin present in archeal cells is a protein that acts as a proton pump that can pump protons across the membrane in the presence of light energy. ATP synthase is an ion pump, molecular motor, and is an enzyme that catalyzes the production of ATP. These two different proteins from the different sources are added to the vesicle, and ATP is produced in the vesicle.

C.

Summary Introduction

To explain: The reasons to defend the friends statement “Why would anybody want to mix vanilla pudding with brake fluid’’ about the new experiment.

Concept introduction:

Bacteriorhodopsin present in archeal cells is a protein that acts as a proton pump that can pump protons across the membrane in the presence of light energy. ATP synthase is an ion pump, molecular motor, and is an enzyme that catalyzes the production of ATP. These two different proteins from the different sources are added to the vesicle, and ATP is produced in the vesicle.