Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 14, Problem 2CHI
Describe the
- a. A strain with a mutation in the gene encoding the lac repressor; the mutant repressor cannot bind allolactose.
- b. A strain with a mutation in the gene encoding CAP; the mutant form of CAP binds but cannot release cAMP.
- c. A strain in which the Shine-Dalgarno sequence has been deleted from the gene encoding adenyl cyclase.
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Chapter 14 Solutions
Prescott's Microbiology
Ch. 14.2 - MICRO INQUIRY In what way is on inducer molecule...Ch. 14.2 - MICRO INQUIRY Is allolactose a corepressor or...Ch. 14.2 - Retrieve, Infer, Apply Many genes and operons are...Ch. 14.2 - Retrieve, Infer, Apply What are induction and...Ch. 14.2 - Retrieve, Infer, Apply Using figure 14.4 as a...Ch. 14.3 - MICRO INQUIRY How does this attenuation respond to...Ch. 14.4 - MICRO INQUIRY How does inhibition of translation...Ch. 14.4 - Prob. 1RIACh. 14.4 - Retrieve, Infer, Apply Describe how attenuation...Ch. 14.4 - Retrieve, Infer, Apply What are translational...
Ch. 14.4 - Retrieve, Infer, Apply How are translational...Ch. 14.4 - Prob. 5RIACh. 14.5 - MICRO INQUIRY Relative to each promoter, where...Ch. 14.5 - MICRO INQUIRY For what other compounds would you...Ch. 14.5 - Prob. 3MICh. 14.5 - MICRO INQUIRY Why does V. harveyi make three...Ch. 14.5 - Prob. 5MICh. 14.5 - Prob. 6MICh. 14.5 - Prob. 1.1RIACh. 14.5 - Retrieve, Infer, Apply What is diauxic growth?...Ch. 14.5 - Retrieve, Infer, Apply Describe the events that...Ch. 14.5 - Retrieve, Infer, Apply E. coli has two phosphate...Ch. 14.5 - Prob. 1.5RIACh. 14.5 - Prob. 1.6RIACh. 14.5 - Prob. 2.1RIACh. 14.5 - Retrieve, Infer, Apply Why might bacteria use...Ch. 14.5 - Prob. 2.3RIACh. 14.5 - Prob. 2.4RIACh. 14 - Attenuation affects anabolic pathways, whereas...Ch. 14 - Describe the phenotype of the following E. coli...Ch. 14 - Prob. 3CHICh. 14 - What would be the phenotype of a B. subtilis...Ch. 14 - Propose a mechanism by which a cell might sense...Ch. 14 - Neisseria meningitidis, commonly called...
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- There is Hyaluronic acid synthesis occuring in Group X Strep and it is controlled by an operon with 3 genes, called hasXYZ. Based on the 3-line diagram model, a. How many ribosome binding sites are there for the protein? b. How many promoters are there for the genes? c. How many start codons are there for the protein? d. How many RNA Polymerase binding locations are there for the genes? e. How many proteins will be fully functional? f. How many mRNA strands are made?arrow_forwardThe cyanobacterium Oscillatoria sancta appears reddish-brown when grown under green light but alters its gene expression patterns and becomes blue-green when grown under red light. Explain this observation.arrow_forwarda. Would you expect a cell to continue or to stopdividing at a nonpermissive high temperature if itis a temperature-sensitive Ras mutant whose protein product is fixed in the GTP-bound form atnonpermissive temperature?b. What would you expect if you had a temperaturesensitive mutant in which the Ras protein staysin the GDP-bound form at high temperature?arrow_forward
- A mutation occurred on the lacI (repressor) gene resulting in a constant production of beta-galactosidase and permease, even in the absence of lactose. Provide an explanation for the effect of this mutation on the repressor protein moleculearrow_forwardA strain of bacteria possesses a temperature-sensitive mutation in the gene that encodes the rho subunit. At high temperatures, rho is not functional. When these bacteria are raised at elevated temperatures, which of the following effects would you expect to see? Explain your reasoning for accepting or rejecting each of these five options. a. Transcription does not take place. b. All RNA molecules are shorter than normal. c. All RNA molecules are longer than normal. d. Some RNA molecules are longer than normal. e. RNA is copied from both DNA strands.arrow_forwardThe above answer did not address the question in the sense that the answer did not indicate which protein involved in the pathways could be measured in the nucleus of cells and be confident in the results? I reiterate the question as follows: You want to do a single Western blot to measure changes in signaling activity, regardless of which pathway is being activated. For which protein involved in these pathways could you measure the levels in the nucleus of cells and be confident in your results? Why?arrow_forward
- Which option is true when considering the regulation of cAMP and the Lac operon. If bacteria are in a medium containing low glucose, but suddenly glucose concentration in the medium increases by ten times, then we expect that cAMP...... a) Will increase inside the cell. b) Will not increase inside the cell.arrow_forwardInterpret this: what does this say about b-galactosidase production? Does Lac mutant exhibit it?arrow_forwardBoth ADA-SCID and type I diabetes are diseases based on lack of a particular protein. Why has the pioneering work on gene therapy focused on SCID instead of on diabetes?arrow_forward
- Repressors are inactivated either by interaction with a small-molecule inducer or by proteolytic cleavage. Why is it advantageous for a repressor like the lac repressor to be inactivated by binding to allolactose rather than by proteolytic cleavage?arrow_forwardList and explain two ways that this transcription factor (HIF-1α) relates to glucose metabolism.arrow_forwardExplain why (a) inactivation of the O2 or O3 sequence of the lac operon causes only a twofold loss in repression, and (b) inactivation of both O2 and O3 reduces repression ∼70-fold.arrow_forward
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