BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 14, Problem 2S
In the Meselson-Stahl experiment, a control experiment was done to show that the hybrid bands after one round of replication were in fact two complete strands, one heavy and one light. Using the same experimental setup as detailed in the text, how can this be addressed?
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In the Meselson–Stahl experiment, which of the three modes of replication could be ruled out after one round of replication? after two rounds?
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Chapter 14 Solutions
BIOLOGY
Ch. 14.1 - Describe the experiments of Griffith and Avery.Ch. 14.1 - Evaluate the evidence for DNA as genetic material.Ch. 14.2 - Explain how the WatsonCrick structure rationalized...Ch. 14.2 - Prob. 2LOCh. 14.3 - Prob. 1LOCh. 14.3 - Prob. 2LOCh. 14.4 - Prob. 1LOCh. 14.4 - Prob. 2LOCh. 14.4 - Diagram the functions found at the replication...Ch. 14.5 - Compare eukaryotic replication with prokaryotic.
Ch. 14.5 - Prob. 2LOCh. 14.5 - Prob. 3LOCh. 14.6 - Prob. 1LOCh. 14.6 - Prob. 2LOCh. 14 - Prob. 1DACh. 14 - Prob. 2DACh. 14 - Prob. 1IQCh. 14 - Prob. 2IQCh. 14 - How does the structure of eukaryotic genomes...Ch. 14 - Prob. 4IQCh. 14 - Prob. 1UCh. 14 - Which of the following is NOT a component of DNA?...Ch. 14 - Chargaff studied the composition of DNA from...Ch. 14 - The bonds that hold two complementary strands of...Ch. 14 - Prob. 5UCh. 14 - Prob. 6UCh. 14 - Which of the following is NOT pan of the...Ch. 14 - If one strand of a DNA is 5 ATCGTTAAGCGAGTCA 3,...Ch. 14 - Hershey and Chase used radioactive phosphorus and...Ch. 14 - The Meselson and Stahl experiment used a density...Ch. 14 - Prob. 4ACh. 14 - If the activity of DNA ligase was removed from...Ch. 14 - Successful DNA synthesis requires all of the...Ch. 14 - The synthesis of telomeres a. uses DNA polymerase,...Ch. 14 - When mutations that affected DNA replication were...Ch. 14 - Prob. 1SCh. 14 - In the Meselson-Stahl experiment, a control...Ch. 14 - Enzyme function is critically important for the...
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- he Meselson and Stahl experiment provided conclusive evidence for the semiconservative replication of DNA in E. coli. What pattern of bands would they have observed in a CsCl gradient after one generation if replication was conservative? a heavy, a light and an intermediate band one heavy and one light band (no intermediate) one intermediate band one light band one heavy bandarrow_forwardWhat observation in the Meslson and Stahl experiment to decipher the DNA replication model distinguishes the semi-conservative from the dispersive mode of replication? Explain.arrow_forwardThe Meselson-Stahl experiment provided strong evidence that DNA replication was conservative, by alternately growing bacteria in medium with heavy 15N and light 14N. If DNA replication were dispersive, what result would Meselson and Stahl have observed after the first round of DNA replication in light nitrogen? Group of answer choices Two bands, one at the location for pure 15N and one at the location for pure 14N. One band, located half way between the locations for pure 15N and pure 14N. Two bands, one at the location for pure 15N and one located halfway between the locations for pure 15N and pure 14N. None of these Three bands, one at the location for pure 15N, one at the location for pure 14N, and one at a location halfway between.arrow_forward
- If the four deoxynucleotides showed nonspecific basepairing (A to C, A to G, T to G, and so on), would theunique information contained in a gene be maintainedthrough round after round of replication? Explainarrow_forwardWhat would be a primer sequence synthesized from the following DNA sequence: 5'-ACGTG-3'? out of 5'-GTGCA-3', 5'-CACGT-3', 5'-CACGU-3', 5'-TGCAC-3', or 5'-ACGTG-3'?arrow_forwardAflatoxin B1 is a highly mutagenic and carcinogenic compound produced by certain fungi that infect crops such as peanuts. Aflatoxin is a large, bulky molecule that chemically bonds to the base guanine (G) to form the aflatoxin-guanine adduct that is pictured below. In the figure below, the aflatoxin is orange, and the guanine base is purple. This adduct distorts the DNA double helix and blocks replication. a. What type(s) of DNA repair system is (are) most likely to be involved in repairing the damage caused by exposure of DNA to aflatoxin B1? b, Recent evidence suggests that the adduct of guanine and aflatoxin B1 can attack the bond that connects it to deoxyribose; this liberates the adducted base, forming an apurinic site. How does this new information change your answer to part (a)?arrow_forward
- In the Meselson Stahl experiment differentiating the possible modes of replication, how many rounds of replication were needed to determine that the following modes of replication were not used in E. coli? two rounds of replication for conservative; two rounds of replication for dispersive one round of replication for conservative; two rounds of replication for dispersive two rounds of replication for conservative; one round of replication for dispersive one round of replication for conservative; one round of replication for dispersive none of the above is correctarrow_forwardWhat is meant by semi conservative replication? How did Meselson and Stahl prove it experimentally?arrow_forwardConsider the experiment conducted by Meselson and Stahl in which they used 14N and 15N in cultures of E. coli and equilibrium density gradient centrifugation. Draw pictures to represent the bands produced by bacterial DNA in the centrifuge tube before the switch to medium containing 14N and after one, two, and three rounds of replication in that medium. Use separate sets of drawings to show the bands that would appear if replication were (a) semiconservative; (b) conservative; (c) dispersive.arrow_forward
- What results would be expected in the experiment outlined in Figure if, during replication, all the original histone proteins remained on one strand of the DNA and new histones attached to the other strand?arrow_forwardWith regard to the repair of double-strand breaks, what are the advantages and disadvantages of homologous recombination repair versus nonhomologous end joining?arrow_forwardWhat is the role of a primer in DNA replication? What would happen if you forgot to add a primer in a tube containing the reaction mix for a DNA sequencing reaction?arrow_forward
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