BIOLOGY (LOOSELEAF)-W/CONNECT
12th Edition
ISBN: 9781260692181
Author: Raven
Publisher: MCG
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Chapter 14, Problem 3A
The Meselson and Stahl experiment used a density label to be able to
a. determine the directionality of
b. differentially label DNA and protein.
c. distinguish between newly replicated and old strands.
d. distinguish between replicated DNA and RNA primers.
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The Meselson and Stahl experiment used a density label toa. determine the directionality of DNA replication.b. differentially label DNA and protein.c. distinguish between newly replicated and old strands.d. distinguish between replicated DNA and RNA primers.
Which of the following statements BEST DESCRIBES the main findings of the Meselson-Stahl experiment?
A.
DNA can be separated using centrifugation
B.
The semiconservative model of DNA replication is more accurate than the dispersive or conservative models of DNA replication
C.
Using 14N in experiments is an effective way of tracking nitrogen molecules
D.
Bacteria grown in the presence of a heavier nitrogen isotope (15N) will replicate at a slower rate than those that utilise a lighter nitrogen isotope (14N)
E.
Both strands of each new DNA double helix are brand new and synthesized from individual nucleotides
In the Meselson-Stahl experiment, what happened after the E. coli was moved to the N14 medium and two rounds of cell division occurred? This result demonstrated that DNA replicated in a semi-conservative manner.
A. The DNA formed one band at the N15 density level and one band at the N14 density level.
B. The DNA formed one band between the N14 and N15 density levels and one band at the N14 density level.
.C. The DNA only formed one band between the N14 and N15 density levels.
Chapter 14 Solutions
BIOLOGY (LOOSELEAF)-W/CONNECT
Ch. 14.1 - Describe the experiments of Griffith and Avery.Ch. 14.1 - Evaluate the evidence for DNA as genetic material.Ch. 14.2 - Explain how the WatsonCrick structure rationalized...Ch. 14.2 - Prob. 2LOCh. 14.3 - Prob. 1LOCh. 14.3 - Prob. 2LOCh. 14.4 - Prob. 1LOCh. 14.4 - Prob. 2LOCh. 14.4 - Diagram the functions found at the replication...Ch. 14.5 - Compare eukaryotic replication with prokaryotic.
Ch. 14.5 - Prob. 2LOCh. 14.5 - Prob. 3LOCh. 14.6 - Prob. 1LOCh. 14.6 - Prob. 2LOCh. 14 - Prob. 1DACh. 14 - Prob. 2DACh. 14 - Prob. 1IQCh. 14 - Prob. 2IQCh. 14 - How does the structure of eukaryotic genomes...Ch. 14 - Prob. 4IQCh. 14 - Prob. 1UCh. 14 - Which of the following is NOT a component of DNA?...Ch. 14 - Chargaff studied the composition of DNA from...Ch. 14 - The bonds that hold two complementary strands of...Ch. 14 - Prob. 5UCh. 14 - Prob. 6UCh. 14 - Which of the following is NOT pan of the...Ch. 14 - If one strand of a DNA is 5 ATCGTTAAGCGAGTCA 3,...Ch. 14 - Hershey and Chase used radioactive phosphorus and...Ch. 14 - The Meselson and Stahl experiment used a density...Ch. 14 - Prob. 4ACh. 14 - If the activity of DNA ligase was removed from...Ch. 14 - Successful DNA synthesis requires all of the...Ch. 14 - The synthesis of telomeres a. uses DNA polymerase,...Ch. 14 - When mutations that affected DNA replication were...Ch. 14 - Prob. 1SCh. 14 - In the Meselson-Stahl experiment, a control...Ch. 14 - Enzyme function is critically important for the...
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- Which of the following best describes the process of DNA seqencing. a. DNA is seperated on a gel and the different bands are labled with flouroscent nucleotides and scanned with a laser. b. A laser is used to flurorescently label the nucleotides present with in the DNA , the DNA is run on a gel and then the DNA is droken into fragments c. Nucleotides are scanned with a laser and incrprorated into the DNA that has been seperated on a gel and then DNA is amplified with PCR. d. fragments of DNA are produced in a reaction that lables them with any of four different fluroscent dyes and the fragmented then are run on a gel and scanned with laser e. DNA is broken down into its constituents nucleotides and the nucleotides are then run on a gel and purified with a laserarrow_forwardWhat is a recombinant DNA? Select one: a. During meiosis, sister chromatids cross over to produce daughter cells with recombinant DNA. b. Any genome that is non-parental is called a recombinant DNA. c. New DNA sequences are inserted into a genome to produce recombinant DNA. d. Bacterial plasmid with sticky ends are referred to as the recombinant DNA.arrow_forward(b) Use a drawing to illustrate the principle of DNA gel electrophoresis. +arrow_forward
- What was the significance of Meselson and Stahl’s experiments on DNA replication using the heavy isotope of Nitrogen? A. telomerase was identified as the molecule responsible for solving the end replication problem of eukaryotic chromosomes B. the existence of lagging strand synthesis was proven. C. the rate of DNA synthesis by DNA polymerase was measured D. the processivity of DNA polymerase was established E. the semi-conservative mode of DNA replication was confirmedarrow_forwardHow does E. coli determine which nucleotide to "fix" in a base mismatch? The appropriate DNA repair system: A.recognizes fully methylated DNA and replaces the purine. B. recognizes partially methylated DNA and replaces the pyrimdine. C. Recognizes partially methylated DNA and fixes the methylated strand D. Recongnizes partially methylated DNA and fixes the nonmethylated strand E. Makes no distiction between strands, and therefore fixes the wrong base 50% of the timearrow_forwardIn Noll’s experiment to test the beads-on-a-string model, exposure of nuclei to a low concentration of DNase I resulted ina. a single band of DNA with a size of approximately 200 bp.b. several bands of DNA in multiples of 200 bp.c. a single band of DNA with a size of 100 bp.d. several bands of DNA in multiples of 100 bparrow_forward
- A.) Base excision repair requires polymerases. B.) In DNA repair by excision, the non-damaged strand is used as a template for a new strand of DNA. a. Statement A is correct b. Statement B is correct c. Both A and B are correct d. Both A and B are incorrectarrow_forwardWhich one of the following statements is true? a. Dideoxynucleotides signal the end of DNA replication in a cell b. None of the provided answers are true c. The DNA sequence determined by an autoradiogram should be identical to the template strand DNA sequence d. The probe hybridization solution used in the Sothern blot technique is approximately pH 10.0 e. The transfer buffer used in the Southern blot technique is approximately pH 7.0arrow_forwardSuppose the experiment of Meselson and Stahl was performed on a sample of 8 cells, each containing one copy of its circular double-stranded DNA genome, and that had been growing on normal 14N medium. You then grew the cells for 3 generations in medium containing 15N. The outcome would be A) 8 cells with single-stranded DNA molecules with 14N, and 24 cells with single-stranded DNA molecules with 15N. B) 16 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 48 cells with double-stranded DNA molecules with 15N. C) 8 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 24 cells with double-stranded DNA molecules with 15N. D) 8 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 32 cells with double-stranded DNA molecules with 15N. E) 65 cells with single-stranded DNA molecules with 15N.arrow_forward
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