Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 14, Problem 6TYK
Summary Introduction
Introduction:
Meselson and Stahl experiment explained the replication of DNA (deoxyribonucleic acid) to be semiconservative. The bacteria was grown in a medium containing a heavy isotope of nitrogen and then the bacteria was reproduced in a medium having a light isotope of nitrogen. After each generation, the DNA was centrifuged, in order to separate the DNA molecules on the basis of its density. The result demonstrated a semiconservative replication of the DNA.
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In the Meselson-Stahl experiment, what happened after the E. coli was moved to the N14 medium and two rounds of cell division occurred? This result demonstrated that DNA replicated in a semi-conservative manner.
A. The DNA formed one band at the N15 density level and one band at the N14 density level.
B. The DNA formed one band between the N14 and N15 density levels and one band at the N14 density level.
.C. The DNA only formed one band between the N14 and N15 density levels.
What is the function of resolvase in recombination?
a. It unwinds double-stranded DNA.
b. It allows a single DNA strand to invade a DNA helix.
c. It displaces one of the original DNA strands during branch migration.
d. It cleaves the Holliday structure.
The diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction?
Group of answer choices
a. The number of copies triples (or triplicates).
b. The number of copies does not change.
c. The number of copies quadruples (or quadruplicates).
d. The number of copies doubles (or duplicates).
e. The number of copies halves.
Chapter 14 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 14.1 - Prob. 1SBCh. 14.2 - Prob. 1SBCh. 14.2 - Prob. 2SBCh. 14.2 - Prob. 3SBCh. 14.2 - Prob. 4SBCh. 14.3 - What is the importance of complementary base...Ch. 14.3 - Why is a primer needed for DNA replication? How is...Ch. 14.3 - DNA polymerase III and DNA polymerase I are used...Ch. 14.3 - Prob. 4SBCh. 14.4 - Why is a proofreading mechanism important for DNA...
Ch. 14 - Working on the Amazon River, a biologist isolated...Ch. 14 - Prob. 2TYKCh. 14 - Pyrimidines built from a single carbon ring are:...Ch. 14 - Which of the following statements about DNA...Ch. 14 - Which of the following statements about DNA is...Ch. 14 - Prob. 6TYKCh. 14 - Prob. 7TYKCh. 14 - Prob. 8TYKCh. 14 - Prob. 9TYKCh. 14 - Prob. 10TYKCh. 14 - Discuss Concepts Eukaryotic chromosomes can be...Ch. 14 - Prob. 12TYKCh. 14 - Prob. 13TYKCh. 14 - Discuss Concepts During replication, an error...Ch. 14 - Design an Experiment Design an experiment using...Ch. 14 - Prob. 16TYKCh. 14 - Prob. 1ITDCh. 14 - Prob. 2ITD
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- Why is DNA replication considered semiconservative? A. Both strands of each replicating DNA molecules are conserved. B. One molecule consists of the old strands and the other DNA molecule is entirely new. C. One strand of each replicating DNA molecule is conserved and the other strand is newly synthesized. D. Both strands of each replicating DNA molecule are new.arrow_forwardWhat is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forward(A) After three cycles of PCR, how many DNA molecules are present that correspond precisely to the desired amplification product? (B) What about after 5 cycles. Assume that we started with one molecule in each case, and that the reaction is perfectly efficient.arrow_forward
- A. In NOT more than 200 words, explain how the double-helical structure of DNA suggests a mechanism for DNA replication? B. In NOT more than 200 words, explain the special mechanism used to replicate chromosome ends?arrow_forwardBase analogs are mutagenic because of which characteristic? a. They produce changes in DNA polymerase that cause it to malfunction. b. They distort the structure of DNA. c. They are similar in structure to the normal bases. d. They chemically modify the normal bases.arrow_forwardWhat would be the result if an organism’s telomerase were mutated and nonfunctional? a. No DNA replication would take place. b. The DNA polymerase enzyme would stall at the telomere. c. Chromosomes would shorten with each new generation. d. RNA primers could not be removed.arrow_forward
- Refer to Figure 2 and compare this with the DNA model in Figure 1. a. In what ways are they similar? b. In what ways are they different? c. What is the biological significance of such differences? Why is the DNA referred to as the genetic material?arrow_forwardIf you performed PCR to amplify a gene region, and you began with 20 copies of double stranded genome, after 18 cycles, about how many double stranded copies of that region would you have (in theory)?arrow_forwardThe following diagrams represent DNA molecules that are undergoing replication. Draw in the strands of newly synthesized DNA and identify (a) the polarity of the newly synthesized strands, (b) the leading and lagging strands, (c) Okazaki fragments, and (d) RNA primers.arrow_forward
- The following diagram represents a DNA molecule that is undergoing replication. Draw in the strands of newly synthesized DNA and identify (a) the polarity of the newly synthesized strands, (b) the leading and lagging strands, (c) Okazaki fragments, and (d) RNA primers.arrow_forwardWhat is DNA polymerase? a.An enzyme that carries out DNA replication b.Short, single strand of DNA that base-pairs with a specific DNA sequence c.An enzyme that corrects mutations that arise during the replication of DNA d.An enzyme that seals any gaps that remain between bases of replicating strands of DNAarrow_forwardWhat was the significance of Meselson and Stahl’s experiments on DNA replication using the heavy isotope of Nitrogen? A. telomerase was identified as the molecule responsible for solving the end replication problem of eukaryotic chromosomes B. the existence of lagging strand synthesis was proven. C. the rate of DNA synthesis by DNA polymerase was measured D. the processivity of DNA polymerase was established E. the semi-conservative mode of DNA replication was confirmedarrow_forward
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