ESSENTIALS OF GENETICS MCC BUNDLE >BI<
9th Edition
ISBN: 9781323915370
Author: KLUG
Publisher: PEARSON
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Textbook Question
Chapter 15, Problem 10PDQ
Describe experiments that would confirm whether or not two transcription regulatory molecules act through the mechanism of cooperative binding.
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If the binding activity was purified, suggest two experimental approaches that could be taken to verify that this factor is in fact a transcription factor? Explain.
Tabulate the similarities and differences between factor-dependent and factor-independent transcription termination.
Describe three ways that the function of a regulatory transcription factor can be modulated.
Chapter 15 Solutions
ESSENTIALS OF GENETICS MCC BUNDLE >BI<
Ch. 15 -
CASE STUDY | A mysterious muscular dystrophy
A...Ch. 15 -
CASE STUDY |A mysterious muscular dystrophy
A...Ch. 15 -
CASE STUDY |A mysterious muscular dystrophy
A...Ch. 15 -
HOW DO WE KNOW?
1. In this chapter, we have...Ch. 15 -
2. Review the Chapter Concepts list on p. 280....Ch. 15 - Describe which enzymes are required for lactose...Ch. 15 - Contrast positive versus negative regulation of...Ch. 15 -
5. Both attenuation and riboswitches rely on...Ch. 15 - For the lac genotypes shown in the accompanying...Ch. 15 -
7. For the genotypes and conditions (lactose...
Ch. 15 -
8. The locations of numerous lacI– and lacIs...Ch. 15 - Explain why catabolite repression is used in...Ch. 15 - Describe experiments that would confirm whether or...Ch. 15 - Predict the level of genetic activity of the lac...Ch. 15 - Predict the effect on the inducibility of the lac...Ch. 15 -
13. Describe the role of attenuation in the...Ch. 15 -
14. In a theoretical operon, genes A, B, C, and D...Ch. 15 - A bacterial operon is responsible for production...Ch. 15 - A marine bacterium is isolated and is shown to...Ch. 15 -
17. Why is gene regulation more complex in a...Ch. 15 -
18. List and define the levels of eukaryotic gene...Ch. 15 -
19. Distinguish between the cis-acting regulatory...Ch. 15 - Prob. 20PDQCh. 15 - Compare the control of gene regulation in...Ch. 15 - Many eukaryotic promoter regions contain CAAT...Ch. 15 -
23. What is RNA-induced gene silencing in...Ch. 15 - Although it is customary to consider...Ch. 15 - DNA methylation is commonly associated with a...Ch. 15 - The interphase nucleus appears to be a highly...Ch. 15 - It has been estimated that at least two-thirds of...
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- How can you show that σ (sigma factor) does not really accelerate the rate of transcription elongation?arrow_forwardIdentify three key ways that TrXG genes promote transcription with respect to: Mediator Complex Structurearrow_forwardDescribe four mechanisms that result in the generation ofantigen receptor diversity.arrow_forward
- Outline the essential features of the three basic phases of transcription. How might each of them be targeted to regulate gene expression? Which of the three phases is the most common target of regulatory mechanisms?arrow_forwardExplain how HOTAIR plays a role in the transcriptional regulationof particular genes.arrow_forwardExplain how a gene knockdown reagent, such as RNAi or a morpholino, could interfere with expression of a gene without affecting the rate of transcription of the target gene.arrow_forward
- What is the experimental basis for concluding that puffs in polytene chromosomes and loops in lampbrush chromosomes are areas of intense transcription of RNA?arrow_forwardExplain Molecular Mechanisms of Transcription Repression and Activation.arrow_forwardDescribe the function of eukaryotic enhancers in transcription.arrow_forward
- Through alternative splicing, eukaryotes (a) reinforce gene inactivation (b) prevent transcription of heterochromatin (c) produce related but different proteins in different tissues (d) amplify genes to meet the requirement of high levels of a gene product (e) bind transcription factors to enhancers to activate transcriptionarrow_forwardDescribe the three models for RNAP active-center translocation during transcription initiation?arrow_forwardBriefly name and explain five mechanisms by which transcription factors activate transcription, with specific reference to targets in the basal transcription complexarrow_forward
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