ESSENTIALS OF GENETICS MCC BUNDLE >BI<
9th Edition
ISBN: 9781323915370
Author: KLUG
Publisher: PEARSON
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Textbook Question
Chapter 15, Problem 25PDQ
DNA methylation is commonly associated with a reduction of transcription. The following data come from a study of the impact of the location and extent of DNA methylation on gene activity in human cells. A bacterial gene, luciferase, was cloned next to eukaryotic promoter fragments that were methylated to various degrees, in vitro. The chimeric plasmids were then introduced into tissue culture cells, and the luciferase activity was assayed. These data compare the degree of expression of luciferase with differences in the location of DNA methylation(Irvine et al., 2002. Mol and Cell Biol 22: 6689–6696). What general conclusions can be drawn from these data?
DNA Segment | Patch Size of Methylation (kb) |
Number of Methylated CpGs | Relative Luciferase Expression |
Outside transcription | 0.0 | 0 | 490
|
unit (0–7.6 kb away) | 2.0 | 100 | 290
|
3.1 | 102 | 250
|
|
12.1 | 593 | 2
|
|
Inside transcription unit | 0.0 | 0 | 490
|
1.9 | 108 | 80
|
|
2.4 | 134 | 5
|
|
12.1 | 593 | 2
|
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Chapter 15 Solutions
ESSENTIALS OF GENETICS MCC BUNDLE >BI<
Ch. 15 -
CASE STUDY | A mysterious muscular dystrophy
A...Ch. 15 -
CASE STUDY |A mysterious muscular dystrophy
A...Ch. 15 -
CASE STUDY |A mysterious muscular dystrophy
A...Ch. 15 -
HOW DO WE KNOW?
1. In this chapter, we have...Ch. 15 -
2. Review the Chapter Concepts list on p. 280....Ch. 15 - Describe which enzymes are required for lactose...Ch. 15 - Contrast positive versus negative regulation of...Ch. 15 -
5. Both attenuation and riboswitches rely on...Ch. 15 - For the lac genotypes shown in the accompanying...Ch. 15 -
7. For the genotypes and conditions (lactose...
Ch. 15 -
8. The locations of numerous lacI– and lacIs...Ch. 15 - Explain why catabolite repression is used in...Ch. 15 - Describe experiments that would confirm whether or...Ch. 15 - Predict the level of genetic activity of the lac...Ch. 15 - Predict the effect on the inducibility of the lac...Ch. 15 -
13. Describe the role of attenuation in the...Ch. 15 -
14. In a theoretical operon, genes A, B, C, and D...Ch. 15 - A bacterial operon is responsible for production...Ch. 15 - A marine bacterium is isolated and is shown to...Ch. 15 -
17. Why is gene regulation more complex in a...Ch. 15 -
18. List and define the levels of eukaryotic gene...Ch. 15 -
19. Distinguish between the cis-acting regulatory...Ch. 15 - Prob. 20PDQCh. 15 - Compare the control of gene regulation in...Ch. 15 - Many eukaryotic promoter regions contain CAAT...Ch. 15 -
23. What is RNA-induced gene silencing in...Ch. 15 - Although it is customary to consider...Ch. 15 - DNA methylation is commonly associated with a...Ch. 15 - The interphase nucleus appears to be a highly...Ch. 15 - It has been estimated that at least two-thirds of...
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