MindTap Biology, 1 term (6 months) Printed Access Card for Starr/Taggart/Evers/Starr's Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN: 9781305269842
Author: Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher: Cengage Learning
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Textbook Question
Chapter 15, Problem 14SQ
Match the method with the appropriate enzyme.
_____ | PCR | a. Taq polymerase |
_____ | cutting DNA | b. DNA ligase |
_____ | cDNA synthesis | c. reverse transcriptase |
_____ | DNA sequencing | d. restriction enzyme |
_____ | pasting DNA | e. DNA polymerase (not Taq) |
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Choose the one answer that fits best. Which statement regarding Molecular Biology is NOT correct (videos)?
a.
Taq Polymerase was isolated from an organism found in Yellowstone Park
b.
Restriction enzymes leave sticky ends
c.
DNA sequencing allows us to read DNA sequences
d.
Restriction enzymes cut DNA at specific sites
e.
EcoRI and HindII are commonly used polymerases
All are true about PCR except
A. Automated PCR machine called a thermal cycler
B. A thermostable DNA polymerase is required
C. Primer extension occurs at 72˚C
D. Annealing involves binding of primer between 90-98˚C
The PCR technique uses (a) heat-resistant DNA polymerase (b) reverse transcriptase (c) DNA ligase (d) restriction enzymes (e) b and c
Chapter 15 Solutions
MindTap Biology, 1 term (6 months) Printed Access Card for Starr/Taggart/Evers/Starr's Biology: The Unity and Diversity of Life (MindTap Course List)
Ch. 15 - _________ cut(s) DNA molecules at specific sites....Ch. 15 - Prob. 2SQCh. 15 - Prob. 3SQCh. 15 - For each species, all ________ in the complete set...Ch. 15 - A set of cells that host various DNA fragments...Ch. 15 - Prob. 6SQCh. 15 - Prob. 7SQCh. 15 - PCR can be used _______. a. to increase the number...Ch. 15 - An individuals set of unique _______ can be used...Ch. 15 - A transgenic organism _______. a. carries a gene...
Ch. 15 - Enhanced Spatial Learning in Mice With an Autism...Ch. 15 - Enhanced Spatial Learning in Mice With an Autism...Ch. 15 - Prob. 3DAACh. 15 - Prob. 4DAACh. 15 - True or false? A transgenic organism can pass a...Ch. 15 - Which of the following can be used to carry...Ch. 15 - Prob. 13SQCh. 15 - Match the method with the appropriate enzyme....Ch. 15 - Match each term with the most suitable...Ch. 15 - Prob. 1CTCh. 15 - Prob. 2CT
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- _____ cut(s) DNA molecules at specific sites. a. DNA polymerase c. Restriction enzymes b. DNA probes d. Reverse transcriptasearrow_forwardChoose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)? a. PCR requires a copy of RNA that serves as a template b. Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA c. Annealing requires cooler temperatures than denaturation d. Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA e. PCR is a quick way of using minute quantities of DNA to create millions of copiesarrow_forward____ cut(s) DNA molecules at specific sites. a. DNA probes b. Restriction enzymes c. DNA polymerase d. DNA ligasearrow_forward
- Which technique rapidly replicates specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reactionarrow_forwardA protein that can cut DNA at specific DNA base sequences is called aa. DNase. c. restriction enzyme.b. DNA ligase. d. DNA polymerase.arrow_forwardMatch the method with the appropriate enzyme.___ PCR a. Taq polymerase___ cutting DNA b. DNA ligase___ cDNA synthesis c. reverse transcriptase___ DNA sequencing d. restriction enzyme___ pasting DNA e. DNA polymerase (not Taq)arrow_forward
- Which type of cells were used to extract the DNA that was sequenced? a. red blood cells c. white blood cells b. intestinal epithelium d. cheek swab What type of mutation caused Nicholas’s disease? a. frameshift c. nonsense b. missense d. insertionarrow_forwardWhich of the following is FALSE about current Sanger dideoxy DNA sequencing procedures? a. Chain termination occurs during synthesis of a new DNA strand. b. Many steps can be automated. c. No DNA is synthesized in the procedure. d. Fluorescent molecules can be used to detect the DNA.arrow_forwardThe function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forward
- Which of the following is involved in recombinant DNA technology? Explain. MULTIPLE CHOICE. Choose only one: a. DNA polymerase b. DNA probes c. Restrition enzymes d. Reverse transcriptasearrow_forward1. (a) Restriction sites are usually ______. Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequences (b) Involves joining a donor DNA fragment of interest to a vector Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequencesarrow_forwardDefinition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only) a. Clone b. Plasmids c. Biotechnology a. PCR Amplification b. Detection c. Modified Trait d. Human Genome e. Genetic Modified Organismarrow_forward
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