Principles of Instrumental Analysis, 6th Edition
Principles of Instrumental Analysis, 6th Edition
6th Edition
ISBN: 9788131525579
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cenage Learning
Question
Book Icon
Chapter 15, Problem 15.14QAP
Interpretation Introduction

Interpretation:

The expression which shows the independence of collision quenching and direct relation to concentration by Fτ ratio should be determined.

Concept introduction:

The quantum efficiency of fluorescence is the ratio of quantum efficiency of fluorescence and the natural lifetime of the excited state in absence of quenching agent.

Blurred answer
Students have asked these similar questions
The magnetogyric ratio of 19F is 2.5177 x 108 T-1 s-1. Calculate the frequency of the nuclear transition in a field of 7.500 T.
An amino acid on the surface of an enzyme was labelled covalently with 1.5-I AEDANS and it is known that the active site contains a tryptophan residue. The fluorescence quantum yield of tryptophan decreased by 15 per cent due to quenching by 1.5-I AEDANS. What is the distance between the active site and the surface of the enzyme?
Consider the quenching of an organic fluorescent species with τ0 = 3.5 ns by a d-metal ion with kQ = 2.5 × 109 dm3 mol−1 s−1. Predict the concentration of quencher required to decrease the fluorescence intensity of the organic species to 75 per cent of the unquenched value.
Knowledge Booster
Background pattern image
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Principles of Instrumental Analysis
Chemistry
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Cengage Learning
Text book image
Principles of Modern Chemistry
Chemistry
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:Cengage Learning