Nutrient gelatin can be incubated at 35°C. What would you have to do to determine hydrolysis after incubation at 35°C?
To determine:
The way by which hydrolysis of gelatin after incubation at 35 degrees Celsius can be determined.
Introduction:
Gelatin is colorless and flavorless ingredient in food that is formed from collagen. In dry state it is brittle, while in liquid state it is kind of gummy. It is commonly used in medicines, paper, cosmetics, vitamin capsules, and photographic films.
Explanation of Solution
When gelatin is incubated at 35 degree Celsius, then to determine the hydrolysis after 35 degrees Celsius, the test tubes containing gelatin must be kept in refrigerator or in ice tub. As it is done, the unhydrolyzed gelatin solidifies again and the hydrolyzed gelatin remains in liquid state.
The hydrolysis of gelatin after incubation at 35 degrees Celsius can be determined by keeping the tubes at low temperatures.
Want to see more full solutions like this?
Chapter 15 Solutions
EBK LABORATORY EXPERIMENTS IN MICROBIOL
- You need to nake 100mL of a 0.2 molar sucrose (C12H22O11) solution. How much sugar do you need to mix with 100mL of water?arrow_forwardWhat is the purpose of acrylamide and bis-acrylamide? in SDS-Page Gelarrow_forwardUpon doing the experiment of Protein Denaturation, what could be observed in the precipitation of organic solvents if ethyl alcohol is added to an albumin solution in a test tube? What color can be observed?arrow_forward
- Why does water separate out from a gel after freezing?arrow_forwardWhich of the following techniques takes advantage of NET CHARGE to purify/characterize proteins? a. Isoelectric focusing b. Hydrophobic chromatography c. Affinity chromatography d. DialysisExplain.arrow_forwardYou have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing arginine at a concentration of 10 micrograms/mL. How much of the stock solution do you add to 1 liter of plate media?arrow_forward
- In a gel filtration chromatography, what type of gel must be used when the protein size is 2500 Da? Explain.arrow_forwardBased on the structure of lipids triacylglycerol, phospholipid, and cholesterol, why are they easily isolated using non-polar solvents?arrow_forwardWhat is the difference of measuring proteins through UV-VIS spectroscopy at 280 nm and at 595 nm with the presence of the Bradford reagent? (note: explain not less than 5 sentences).arrow_forward
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education