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When using pulsed-field gel electrophoresis, mutations in a microbe’s genome will show up as
- a. different pattern of bands.
- b. nonfluorescent bands.
- c. missing bands.
- d. all of these.
Introduction:
The genetic methods and genome projects have concluded that there are various microbes that cannot be cultured and using different genotypic methods. These microbes can be identified by giving precise results over phenotypic methods. One of the methods is genetic fingerprinting.
Answer to Problem 1Q
Correct answer:
The different bands are formed of DNA fragments when the mutated DNA is run under pulsed-filed gel electrophoresis. Therefore, option (a) is correct.
Option (a) is given as “a different pattern of bands”.
Explanation of Solution
Justify the reasons for the correct statement:
The technique named Pulsed-field gel electrophoresis (PFGE) is quite similar to typical genetic fingerprinting but the difference lies in the DNA fragments segmentation, which is comparatively large for typical methods. This process involves the segregation of similar-sized fragments by application of slow alternating voltage sequences from three distinct tracks. The differently sized DNA fragments are formed by alteration of DNA sequences when the restriction enzymes act upon it. The sudden outbreaks of infectious and other infections are interpreted using the PFGE.
Hence, option (a) is correct.
Justify the reasons for the incorrect statements:
Option (b) is given as “nonfluorescent bands”.
No fluorescence labeled probed are not used in this process. The fluorescent and nonfluorescent bands will not be formed in a given scenario. Hence, it is a wrong answer.
Option (c) is given as “missing bands”.
The missing bands will not be formed in the PFGE process. Hence, it is a wrong answer.
Option (d) is given as “all of these”.
No fluorescence labeled probed are not used in this process. The fluorescent and nonfluorescent bands will not be formed in a given scenario. Hence, it is a wrong answer.
Hence, options (b), (c), and (d) are incorrect.
The PFGE process involves the formation of different bands.
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Chapter 15 Solutions
MICROBIOLOGY PRINT UPGRADE
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