Which of the following is not a property of DNA polymerase? a. It adds dNTPs only in the 5' → 3' direction. b. It requires a primer to work. c. It is associated with a sliding clamp only on the leading strand. d. Its exonuclease activity is involved in proofreading.

Question

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Answer 1

Textbook Question

Chapter 15, Problem 1TYK

Which of the following is not a property of DNA polymerase?

a. It adds dNTPs only in the 5' → 3' direction.

b. It requires a primer to work.

c. It is associated with a sliding clamp only on the leading strand.

d. Its exonuclease activity is involved in proofreading.

Expert Solution & Answer

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

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Students have asked these similar questions

A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Which of the following results is he most likely to observe? a. The reaction will work, but at a significantly slower rate. b. The reaction will work, but the product will contain many undesired mutations. c. The reaction will work, but amplify a region that was not his target. d. The reaction will be completely unsuccessfu

Base analogs are mutagenic because of which characteristic? a. They produce changes in DNA polymerase that cause it to malfunction. b. They distort the structure of DNA. c. They are similar in structure to the normal bases. d. They chemically modify the normal bases.

Would it be possible to start synthesizing the daughter DNA strand without assembling the RNA primer first? Why? Why not? A. Yes, because the 3' OH is already present in the DNA strand which will be used as a template. B. No, because the RNA primer which contains the free 5' PO4 in its ribose will not be synthesized by primase. C. Yes, because the 5' PO4 is already present in the DNA strand which will be used as a template. D. No, because the RNA primer which contains the free 3' OH in its ribose has to be synthesized by primase first.

Answer 2

Textbook Question

Chapter 15, Problem 1TYK

Which of the following is not a property of DNA polymerase?

a. It adds dNTPs only in the 5' → 3' direction.

b. It requires a primer to work.

c. It is associated with a sliding clamp only on the leading strand.

d. Its exonuclease activity is involved in proofreading.

Expert Solution & Answer

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

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Answer 3

Textbook Question

Chapter 15, Problem 1TYK

Which of the following is not a property of DNA polymerase?

a. It adds dNTPs only in the 5' → 3' direction.

b. It requires a primer to work.

c. It is associated with a sliding clamp only on the leading strand.

d. Its exonuclease activity is involved in proofreading.

Expert Solution & Answer

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

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Answer 4

Textbook Question

Chapter 15, Problem 1TYK

Which of the following is not a property of DNA polymerase?

a. It adds dNTPs only in the 5' → 3' direction.

b. It requires a primer to work.

c. It is associated with a sliding clamp only on the leading strand.

d. Its exonuclease activity is involved in proofreading.

Expert Solution & Answer

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

Answer 8

Expert Solution & Answer

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

Introduction:

DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.

Answer 12

Answer to Problem 1TYK

Correct answer:

The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.

Answer 13

Explanation of Solution

Explanation/Justification for the correct answer:

Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.

Explanation for incorrect answers:

Option (a) is given as adding of dNTPs only in the 5’→3’ direction. The DNA polymerase adds the dNTPs on the growing end in the 5’→3’ direction. So, it is an incorrect option.

Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.

Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.

Hence, the options (a), (b), and (d) are incorrect.

Answer 14

Conclusion

DNA polymerase catalyzes the addition of dNTPs in the 5’→3’ direction, it requires a primer for initiation of DNA replication, it has exonuclease proofreading activity, and is associated with sliding clamp on both the leading and lagging strand of DNA.

Answer 15

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Which of the following is not a property of DNA polymerase? a. It adds dNTPs only in the 5' → 3' direction. b. It requires a primer to work. c. It is associated with a sliding clamp only on the leading strand. d. Its exonuclease activity is involved in proofreading.

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Answer to Problem 1TYK

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