Concept explainers
Which of the following is not a property of DNA polymerase?
a. It adds dNTPs only in the 5' → 3' direction.
b. It requires a primer to work.
c. It is associated with a sliding clamp only on the leading strand.
d. Its exonuclease activity is involved in proofreading.
Introduction:
DNA (deoxyribonucleic acid) polymerase is the enzyme responsible for the replication of DNA. The enzymes incorporate the deoxyribonucleotides (dNTPs) into the newly synthesizing DNA molecule from the template DNA. The sliding clamp acts as a processivity promoter factor in the DNA replication. It is crucial for DNA polymerase binding to the template DNA as it prevents the frequent dissociation of the DNA polymerase from the template DNA.
Answer to Problem 1TYK
Correct answer:
The association with the sliding clamp only on the leading strand is not the property of DNA polymerase.
Explanation of Solution
Explanation/Justification for the correct answer:
Option (c) is given as association of DNA polymerase only with sliding clamp on the leading strand. The DNA polymerase is associated with the sliding clamp protein in the leading as well as with the lagging strand. Hence, the Option (c) is correct.
Explanation for incorrect answers:
Option (a) is given as adding of dNTPs only in the
Option (b) is given as primer is needed for DNA polymerase. The primer is an oligonucleotide (short stretch of DNA or RNA [ribonucleic acid]) and is required for the synthesis of DNA using DNA polymerase. So, it is an incorrect option.
Option (d) is given as proofreading is associated with exonuclease activity of DNA polymerase. The enzyme also has an exonuclease activity for proofreading of newly synthesized DNA. These are the property of DNA polymerase. So, it is an incorrect option.
Hence, the options (a), (b), and (d) are incorrect.
DNA polymerase catalyzes the addition of dNTPs in the
Want to see more full solutions like this?
Chapter 15 Solutions
BIOLOGICAL SCIENCE >CUSTOM<
- Which of the following subunits of DNA polymerase has proofreading ability? a. α b. β2 c. θ d. None of the abovearrow_forwardWhich of the following do you think would be true of the sites of the origin of replication (where DNA strands first begin to separate?) Hint: think about which would be the easiest to pull apart. (a) they would be rich in purines (b) they would be rich in A and T sequences (c) they would be rich in pyrimidines (d) they would be rich in G and C sequences (e) they would be rich in A sequencesarrow_forwardWhich of the following describes a Z-DNA helix? a. It is inhibited by methylation of bases b. It is favored by alternating GC sequence c. It has fewer base pairs per turn than the B-DNA d. It is favored in many solutions that are relatively devoid of waterarrow_forward
- DNA polymerase adds nucleotides to Select one: a. the 5′ end, and the leading strand elongates in the 3′ to 5′ direction b. the 3′ end, and the leading strand elongates in the 5′ to 3′ direction c. the 5′ end, and the leading strand elongates in the 5′ to 3′ direction d. the 3′ end, and the leading strand elongates in the 3′ to 5′ directionarrow_forwardDNA strands are anti-parallel and DNA polymerase can only synthesize DNA in a 5' to 3' direction. How does the enzyme synthesize both strands at the same time? A. The leading strand is sythesised in Okazaki fragments B. The lagging strand is synthesised in short Okazaki fragments. C. Only one strand is replicated. D. There are more than one DNA polymerase involved.arrow_forwardThe primer is needed during DNA replication because: A) it provides energy for making the new strand B) actually, primer is NOT needed because DNA polymerase can make DNA "from scratch" C) it helps DNA polymerase to bind to the DNA D) it serves as an existing nucleic acid to which DNA polymerase can add nucleotides to form the new strand E) it serves as the template for making the new strandarrow_forward
- Why must the lagging strand of DNA be replicated in short pieces a. Because of limited space b. To make proofreading of code easier . C. Otherwise, the helix will become distorted . D. The DNA polymerase can synthesize in only one directionarrow_forwardPolymerase Chain Reaction, or PCR, can Group of answer choices A. target a specific region of DNA and cut it out of the rest of the genetic material for further analysis. B. copy the number of copies of a selected region of DNA linearly. C. increase the number of copies of a selected region of DNA exponentially. D. copy the entire genome at least a dozen times.arrow_forwardDNA Polymerase holoenzymes used for DNA replication recognizes A. double-stranded sequences as starting points B. methylated lipids as start points C. acetylated lipids as start points D. single stranded sequences as starting pointsarrow_forward
- DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel? a. The 2000-bp fragment b. The 1000-bp fragment c. The 500-bp fragment d. All will migrate equal distances.arrow_forwardThe polymerase chain reaction uses Taq polymerase rather than a DNA polymerase from E. coli, because Taq polymerasea) introduces fewer errors during DNA synthesis.b) is heat-stable.c) can initiate DNA synthesis at a wider variety of sequences.d) can denature a double-stranded DNA template.e) is easier to obtain.arrow_forwardPlace the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forward
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education