Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
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Chapter 15, Problem 20Q
When PCR is performed “by hand” (not with a prepackaged test kit), the lab environment has to be scrupulously clean. Explain why that might be.
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Why different temperatures are used in a PCR reaction? Please answer at your own words.
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Chapter 15 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 15.1 - Prob. 1AYPCh. 15.1 - Provide a one-sentence description for each of...Ch. 15.2 - Identify factors that may affect the...Ch. 15.2 - Prob. 4AYPCh. 15.2 - NCLEX PREX 1. An RN is training a new staff nurse...Ch. 15.2 - NCLEX PREX 2. A clinical form used to report data...Ch. 15.3 - List at least three different tests that fall in...Ch. 15.3 - Prob. 6AYPCh. 15.3 - Discuss two major drawbacks of phenotypic testing...Ch. 15.3 - Q. What technique in this chapter do most home...
Ch. 15.3 - NCLEX PREX 3. When determining the clinical...Ch. 15.4 - Define the term serology, and explain the...Ch. 15.4 - Identify two immunological diagnostic techniques...Ch. 15.4 - Prob. 2MMCh. 15.5 - Explain why PCR is useful for infectious disease...Ch. 15.5 - Name two examples of techniques that employ...Ch. 15.5 - Explain how whole-genome sequencing can be used...Ch. 15.5 - Prob. 13AYPCh. 15.5 - Prob. 3MMCh. 15.6 - Describe the benefits of lab on a chip...Ch. 15.6 - Prob. 15AYPCh. 15 - When using pulsed-field gel electrophoresis,...Ch. 15 - Explain why it is possible to identify some...Ch. 15 - Serotyping identifies distinct members of the same...Ch. 15 - Prob. 4QCh. 15 - Name some bacterial structures that might be...Ch. 15 - You perform a lumbar puncture on a patient with...Ch. 15 - Which category of diagnosis is represented by...Ch. 15 - Write a paragraph that explains the mycobacterial...Ch. 15 - You inoculated a biochemical test strip with a...Ch. 15 - Prob. 10QCh. 15 - You perform a Kirby-Bauer disk diffusion test to...Ch. 15 - Why might culture conditions affect the results of...Ch. 15 - Which of the following techniques is most likely...Ch. 15 - Why is it more important to use selective media...Ch. 15 - What type of diagnostic method do you think would...Ch. 15 - T or F: Bacterial infection causes the expression...Ch. 15 - Prob. 17QCh. 15 - Prob. 18QCh. 15 - Prob. 19QCh. 15 - When PCR is performed by hand (not with a...Ch. 15 - What kind of a control would be important to run...
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- If you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR tube?arrow_forwardWhich of the following ARE part of a typical PCR reaction mixture? DNA ligase dNTPs (mix of nucleoside tri-phosphates) RNA primers made by primase enzyme template DNA, often from cells collected from hair, cheek swab, or blood 2 DNA primers polymerase enzymearrow_forwardThe laboratory has lost the protocol to follow during the PCR reaction and only give you this. Please fill in the blanks: 94°C _______________ step for _______ minutes 52°C _______________ step for _______ seconds 72°C________________ step for ______ minutes 72°C _______________ step for ______ minutesarrow_forward
- Why is it important to read the product manual when designing your PCR conditions?arrow_forwardIf the PCR analysis of your sample did not work can you suggest why? How can you test if this is true?arrow_forwardIs the given statement that the first step of PCR process involves heating of DNA solution TRUE or FALSE?arrow_forward
- What is the purpose of the first step of PCR when it increases the temperature to 95 C.arrow_forwardA thermocycler is a machine used to heat and cool the PCR sample in a series of three steps per cycle. Describe briefly what happens in each step. a. Step 1 - Heat briefly (for 1 min) to 94 degrees Celsius to ___________ b. Step 2 - Cool (for 1 min) to 50 degrees Celsius to _____________ c. Step 3 - Heat (for 1 min) to 72 degrees Celsius to _____________arrow_forwardA pcr required 5 ul of water To make master for 5 samples you would add 35 ul of water 30 20 25arrow_forward
- What can one do if the annealing temperature was programmed to 65 degrees celcius instead of 50 degrees celcius on the thermocycler during PCR and how would this effect the PCRarrow_forwardsample containing at least one molecule of DNA serves as the starting material for PCR. True Falsearrow_forwardIdentify if the following statements are true or false. Quantitative PCR is also known as the real-time PCR Real-time PCR method uses fluorescent dyes such as acridine orangearrow_forward
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