Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781259709227
Author: Marjorie Kelly Cowan Professor, Heidi Smith
Publisher: McGraw-Hill Education
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Chapter 15, Problem 9Q
You inoculated a biochemical test strip with a patient isolate in a rural clinic you work in, and left it in the incubator overnight. When you came back in you saw that there had been a power outage (with no backup power generator) and the temperature in the incubator was 25°C instead of 37°C. You examine the test strip and you see that the wells display growth. Do you trust the results or not? Why or why not?
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The controls maintain the differential qualities of the test medium
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The controls will ensure that the unknown microbe multiplies on the medium
The controls can show if there was a recent mutation in your unknown microbe
Chapter 15 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 15.1 - Prob. 1AYPCh. 15.1 - Provide a one-sentence description for each of...Ch. 15.2 - Identify factors that may affect the...Ch. 15.2 - Prob. 4AYPCh. 15.2 - NCLEX PREX 1. An RN is training a new staff nurse...Ch. 15.2 - NCLEX PREX 2. A clinical form used to report data...Ch. 15.3 - List at least three different tests that fall in...Ch. 15.3 - Prob. 6AYPCh. 15.3 - Discuss two major drawbacks of phenotypic testing...Ch. 15.3 - Q. What technique in this chapter do most home...
Ch. 15.3 - NCLEX PREX 3. When determining the clinical...Ch. 15.4 - Define the term serology, and explain the...Ch. 15.4 - Identify two immunological diagnostic techniques...Ch. 15.4 - Prob. 2MMCh. 15.5 - Explain why PCR is useful for infectious disease...Ch. 15.5 - Name two examples of techniques that employ...Ch. 15.5 - Explain how whole-genome sequencing can be used...Ch. 15.5 - Prob. 13AYPCh. 15.5 - Prob. 3MMCh. 15.6 - Describe the benefits of lab on a chip...Ch. 15.6 - Prob. 15AYPCh. 15 - When using pulsed-field gel electrophoresis,...Ch. 15 - Explain why it is possible to identify some...Ch. 15 - Serotyping identifies distinct members of the same...Ch. 15 - Prob. 4QCh. 15 - Name some bacterial structures that might be...Ch. 15 - You perform a lumbar puncture on a patient with...Ch. 15 - Which category of diagnosis is represented by...Ch. 15 - Write a paragraph that explains the mycobacterial...Ch. 15 - You inoculated a biochemical test strip with a...Ch. 15 - Prob. 10QCh. 15 - You perform a Kirby-Bauer disk diffusion test to...Ch. 15 - Why might culture conditions affect the results of...Ch. 15 - Which of the following techniques is most likely...Ch. 15 - Why is it more important to use selective media...Ch. 15 - What type of diagnostic method do you think would...Ch. 15 - T or F: Bacterial infection causes the expression...Ch. 15 - Prob. 17QCh. 15 - Prob. 18QCh. 15 - Prob. 19QCh. 15 - When PCR is performed by hand (not with a...Ch. 15 - What kind of a control would be important to run...
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- What is this test? What coated the bottom of the wells? What were we testing for? How did we know it was positive? How did we know the test was valid? How did we stop the reaction? How did you know you did a good job micropipetting your samples?arrow_forwardBriefly explain how you could use the color of the Coomassie assay sample solution to determine if anunknown sample needs to be diluted in order to measure the sample’s concentrationarrow_forwardWhat is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of an E-test strip.arrow_forward
- Your instructor asks you to isolate and identify the organisms in an unknown culture. You find that the culture contains two gramnegative bacilli that produce swarming colonies. What biochemical test would you use to identify the bacilli? Justify your answer.arrow_forwardHow could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be to the point.arrow_forwardIf a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe growing within the zone of inhibitionfor a drug, what should her conclusion be?arrow_forward
- In the “sandwich” ELISA technique, what is the function of the capture antibody? In the “sandwich” ELISA technique, what is the function of the capture antibody? To link an enzyme and the molecule of interest To convert a substrate from colorless to blue To block any “open spaces” on the well before adding the samples that potentially contain the molecule of interest To concentrate the molecule of interestarrow_forwardIn the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?arrow_forwardWhat is the purpose of the blank test in the experiments?arrow_forward
- If you wanted to use PCR to assess microbial activity, what type of PCR would you use and why?arrow_forwardWhich among the following demonstrates the difference between Native Polyacrylamide Gel Electrophoresis (PAGE) and SDS-PAGE? Group of answer choices Native PAGE has a denaturation step whereas SDS-PAGE does not have a denaturation step. Native PAGE is based on both the charge and size whereas SDS-PAGE is only based on size. Native PAGE does not have a denaturation step whereas SDS-PAGE has a denaturation step. Native PAGE is based only on charge whereas SDS-PAGE is based on both charge and size.arrow_forwardIn this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mLarrow_forward
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