A.
To predict: The membrane orientation of a protein that is made with an uncleaved, internal signal sequence but does not contain a stop-transfer sequence.
Concept introduction:
Transmembrane proteins are those proteins that remain embedded in the ER membrane and are not released to the ER lumen. The N-terminal signal sequence starts the transfer process, and it is stopped by a hydrophobic amino acid sequence called the stop-transfer sequence. The translocation channel will release the growing polypeptide chain sideways to bilayer and N-terminal is cleaved off. A α-helical membrane-spanning section is formed that anchors the proteins to the membrane.
B.
To predict: The membrane orientation of a protein that is synthesized with an N-terminal cleaved signal sequence followed by a stop-transfer sequence, followed by a start-transfer sequence.
Concept introduction:
Transmembrane proteins are those proteins that remain embedded in the ER membrane and are not released to the ER lumen. The N-terminal signal sequence starts the transfer process, and it is stopped by a hydrophobic amino acid sequence called the stop-transfer sequence. The translocation channel will release the growing polypeptide chain sideways to bilayer and N-terminal is cleaved off. A α-helical membrane-spanning section is formed that anchors the proteins to the membrane.
C.
To examine: What arrangement of signal sequences would enable the insertion of a multi-pass protein with an odd number of transmembrane segments.
Concept introduction:
Transmembrane proteins are those proteins that remain embedded in the ER membrane and are not released to the ER lumen. The N-terminal signal sequence starts the transfer process, and it is stopped by a hydrophobic amino acid sequence called the stop-transfer sequence. The translocation channel will release the growing polypeptide chain sideways to bilayer and N-terminal is cleaved off. A α-helical membrane-spanning section is formed that anchors the proteins to the membrane.
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