Biology: The Unity and Diversity of Life (MindTap Course List)
15th Edition
ISBN: 9781337408332
Author: Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher: Cengage Learning
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Chapter 15, Problem 8SQ
Summary Introduction
Introduction: Polymerase chain reaction (PCR) is a technique that is used to amplify a deoxyribonucleic acid (DNA) sample to generate its several thousand or million copies. It is one of the most applied technique in molecular biology.
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PCR can be utilized _______
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PCR can be used______ . a. as a cloning vector b. in DNA profiling c. to modify a human genome
Definition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only)
a. Clone
b. Plasmids
c. Biotechnology
a. PCR Amplification
b. Detection
c. Modified Trait
d. Human Genome
e. Genetic Modified Organism
Chapter 15 Solutions
Biology: The Unity and Diversity of Life (MindTap Course List)
Ch. 15 - Enhanced Spatial Learning Ability in Mice...Ch. 15 - Enhanced Spatial Learning Ability in Mice...Ch. 15 - Prob. 3DAACh. 15 - Prob. 4DAACh. 15 - _________ cut(s) DNA molecules at specific sites....Ch. 15 - Prob. 2SQCh. 15 - Prob. 3SQCh. 15 - For each species, all ________ in the complete set...Ch. 15 - A set of cells that host various DNA fragments...Ch. 15 - Prob. 6SQ
Ch. 15 - Prob. 7SQCh. 15 - Prob. 8SQCh. 15 - Prob. 9SQCh. 15 - Prob. 10SQCh. 15 - A transgenic organism _______. a. carries a gene...Ch. 15 - True or false? Some transgenic organisms can pass...Ch. 15 - Taq polymerase is used for PCR because it _______....Ch. 15 - _____ can correct a genetic defect in an...Ch. 15 - Match each term with the most suitable...Ch. 15 - Match the method with the appropriate enzyme....Ch. 15 - Prob. 1CTCh. 15 - Restriction enzymes in bacterial cytoplasm cut...Ch. 15 - The results of a paternity test using short tandem...
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- PCR can be used ______. a. as a cloning vector b. in DNA profiling c. to modify a human genomearrow_forwardAll are true about PCR except A. Automated PCR machine called a thermal cycler B. A thermostable DNA polymerase is required C. Primer extension occurs at 72˚C D. Annealing involves binding of primer between 90-98˚Carrow_forwardRegarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forward
- Which process consists of modifying an individual’s genome? a. DNA profiling b. SNPing c. genetic engineering d. DNA hybridization e. PCRarrow_forwardForensics a. The importance of recombinant DNA technology in Forensics b. Potential products produced and processes involved in forensics (at least 5 examples in each field). c. The downside/ disadvantages of recombinant DNA technology in forensicsarrow_forwardDefinition of Terms( This is all about Applications of Recombinant DNA) a. Clone b. Plasmids c. Biotechnology d. PCR Amplification e. Detection f. Modified Trait g. Human Genome h. Genetic Modified Organismarrow_forward
- A) What are the three main steps involved in PCR? Include temperatures and descriptions of each step. B) Explain why RFLP can produce many bands on an electrophoresis gel and PCR (one set of primers) will only produce one or two bands on a gel for the same genome.arrow_forwardScientists can distinguish between DNA of different individuals, thus making this information useful in criminal investigations. The technique used is called __________. a. DNA fingerprinting b. CRISPR/Cas9 c. fluorescent in situ hybridization d. PCRarrow_forwardThe PCR technique uses (a) heat-resistant DNA polymerase (b) reverse transcriptase (c) DNA ligase (d) restriction enzymes (e) b and carrow_forward
- a. Explain why the PCR is unlikely to amplify contaminatingbacterial DNA in a sample of human DNA. b. Explain how PCR could be used to pick a gene out of a complexgenome and amplify it.arrow_forwardExplain why the PCR is unlikely to amplify contaminating bacterial DNA in a sample of human DNA.Explain how PCR could be used to pick a gene out of a complex genome and amplify it.arrow_forwardPCR is essential for Select one: A. allowing restriction enzymes to cut DNA. B. making many copies of a small amount of DNA. C. cutting DNA into many small pieces. D. creating recombinant plasmids.arrow_forward
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