Concept explainers
To review:
The inferences that can be made regarding the mechanisms of silencing of each gene.
Given:
The table below represents the gene expression data after the treatment with the inhibitors of the DNA (deoxyribonucleic acid) methylation and histone deacetylation.
Introduction:
Two different compounds AZA and TSA are used to inhibit the DNA methyltransferase and histone deacetylase, respectively. The researchers studied the effects of the inhibitors both combinedly and separately.
The cells in which these genes were silenced were also tested in another experiment. These cells were incubated with the inhibitor. The genes of interest were amplified. The amplified products obtained were proportional to the original mRNA (messenger ribonucleic acid) quantities.
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