LIFE: THE SCIENCE OF BIOLOGY
11th Edition
ISBN: 9781319145125
Author: Sadava
Publisher: MAC HIGHER
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Chapter 16.1, Problem 2R
Summary Introduction
To review:
The comparison of molecular conditions that occur at the lac operon promoter in the absence as well as the presence of lactose.
Introduction:
The operon is generally a cluster of genes containing a single promoter. The operon found in Escherichia coli which encodes three lactose
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Explain the process of attenuation in the trp operon under the following conditions:
(i)
No tryptophan
(ii)
High tryptophan levels
Describe the probable genetic defect that abolishes the sensitivity of the lac operon to the absence of glucose when other metabolic operons continue to be sensitive to the absence of glucose.
Explain why inactivation of the O1 sequence of the lac operator almost completely abolishes repression of the lac operon.
Chapter 16 Solutions
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- Predict a possible phenotype from the following mutations in the Lac Operon: a deletion in "GGAATTGTGAGCGGATAACAATTTCAC."arrow_forwardDraw the structure of lac operon and its action when the following conditions are prevailing in the media: increased glucose, low glucose, higher c-AMP, low c-AMP, higher glucose, and high c-AMP, low glucose and High c-AMP, Higher glucose and higher allolactosearrow_forwardDescribe the role of the CRP–cAMP complex in positiveregulation of the lactose operon in E. coli.arrow_forward
- Explain how the data shown indicate that two operator sites are necessary for repression of the lac operon. What would the results have been if all three operator sites were required for the binding of lac repressor?arrow_forwardExplain the process of attenuation of the trp operon under high tryptophan levels.arrow_forwardWhat conservation laws (P- or S- invariants) can be found in the lac operon regulatory Petri net?arrow_forward
- Supply the words “off" or “on" in the blanks below: In an inducible operon, transcription is normally and must be turned In a repressible operon, transcription is normally and must be turned Briefly describe the overall function of the structural genes in the lac operon. Briefly describe the overall function of the structural genes in the trp operon. For the following descriptions of the bacterial lac operon, determine if lactose is present (P) or absent (A). The repressor is bound to the operator. The repressor is inactive. RNA polymerase transcribes the structural genes. Allolactose is bound to the repressor For the following descriptions of the bacterial trp operon, determine if tryptophan levels are high (H) or low (L). The repressor is bound to the operator. The repressor is inactive. RNA polymerase transcribes the structural genes. Tryptophan is bound to the repressor.arrow_forwardPredict the level of genetic activity of the lac operon as well as the status of the lac repressor and the CAP protein under the cellular conditions listed in the accompanying table. Lactose Glucose (a) - - (b) + - (c) - + (d) + +arrow_forwardExplain why large amounts of transcript result when a bacterium with a lac Operon is in a medium with no glucose and an abundance of lactose.arrow_forward
- Explain the role of CAP and cAMP in the regulation of the lac-operon.arrow_forwardUnder what conditions would you expect to see high levels of expression of the structural genes in the lac operon? Group of answer choices Lactose is absent and glucose is absent Lactose is absent and glucose is present Lactose is present and glucose is present None of these Lactose is present and glucose is absentarrow_forwardIn terms of the efficient use of bacterial resources, explain why it is necessary for lac operon expression to be controlled both by the lac repressor and by a cyclic AMP receptor protein rather than just by the lac repressor alone.arrow_forward
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