Microbiology With Diseases By Body System, Books A La Carte Plus Masteringmicrobiology With Etext -- Access Card Package (4th Edition)
4th Edition
ISBN: 9780321942739
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Textbook Question
Chapter 17, Problem 1VI
Identify the chemicals represented by this artist’s conception of an antibody sandwich ELISA.
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All of the following are used in a direct ELISA, except:
A) Primary enzyme linked antibody
B) Substrate
C) Secondary enzyme linked antibody
D) Primary antibody
If you were using the ELISA to look for the presence of antibodies and the sample gave a negative result, does this mean that there were no antibodies present? Explain.
Why is important to note the Kit / Lot number and expiration date for each kit or tests done from each ELISA kit?
Chapter 17 Solutions
Microbiology With Diseases By Body System, Books A La Carte Plus Masteringmicrobiology With Etext -- Access Card Package (4th Edition)
Ch. 17 - Prob. 1TMWCh. 17 - A diagnostician used an ELISA to show that a...Ch. 17 - To obtain immediate immunity against tetanus, a...Ch. 17 - Which of the following vaccine types is commonly...Ch. 17 - Prob. 3MCCh. 17 - When antigen and antibodies combine, maximal...Ch. 17 - An anti-antibody is used when _________. a. an...Ch. 17 - Prob. 6MCCh. 17 - Prob. 7MCCh. 17 - Prob. 8MC
Ch. 17 - Prob. 9MCCh. 17 - Prob. 10MCCh. 17 - Prob. 11MCCh. 17 - An antiserum is ________. a. an anti-antibody b....Ch. 17 - Prob. 13MCCh. 17 - Prob. 14MCCh. 17 - Prob. 15MCCh. 17 - Prob. 1MTFCh. 17 - Prob. 2MTFCh. 17 - Prob. 3MTFCh. 17 - Modified True/False 4. _________________ ELISA is...Ch. 17 - Prob. 5MTFCh. 17 - Match the characteristic in the first column with...Ch. 17 - Identify the chemicals represented by this artists...Ch. 17 - Prob. 2VICh. 17 - Compare and contrast the Chinese practice of...Ch. 17 - What are the advantages and disadvantages of...Ch. 17 - Compare the advantages and disadvantages of...Ch. 17 - How does precipitation differ from agglutination?Ch. 17 - Explain how a pregnancy test works at the...Ch. 17 - Compare and contrast herd immunity and contact...Ch. 17 - How does nephelometry differ from turbidimetry?Ch. 17 - Is it ethical to approve the use of a vaccine that...Ch. 17 - Which is worse: to use a diagnostic test for HIV...Ch. 17 - Discuss the importance of costs and technical...Ch. 17 - What bodily fluids, in addition to blood serum,...Ch. 17 - Why might a serological test give a false positive...Ch. 17 - Some researchers want to distinguish B cells from...Ch. 17 - Describe three ways by which genetic recombinant...Ch. 17 - How does a toxoid vaccine differ from an...Ch. 17 - Explain why many health organizations promote...Ch. 17 - Contrast a hemagglutination test with a viral...Ch. 17 - Prob. 11CTCh. 17 - Draw a picture showing, at both the molecular and...Ch. 17 - Prob. 1CM
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- . Design an ELISA assay for detecting the presence of anti-HIV antibodies in a patient’s serum sample.arrow_forwardWhat is the difference between an indirect ELISA and a sandwich ELISA? Is rapid antibody testing a form of ELISA?arrow_forwardin an indirect elisa procedure what enzyme is used?arrow_forward
- What is the effect of not adding the primary antibody to the ELISA reaction?arrow_forwardIs rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid antibody testing? Describe how antibodies are used to detect the presence of a specific disease.arrow_forwardWhat are the four steps of an Elisa protocolarrow_forward
- In a direct ELISA, which of the following does the patient provide? A.) Substrate B.) Enzymes C.) Antibodies D.) Antigensarrow_forwardExplain why a semi-log plot should be used for determining antigen concentration by ELISA.arrow_forwardWhy do some tests for antibody in serum (such as for HIV and syphilis)require backup verification with additional tests at a later date?arrow_forward
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