BIOLOGY
12th Edition
ISBN: 9781264839698
Author: Raven
Publisher: MCG CUSTOM
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Textbook Question
Chapter 17, Problem 2U
What is the basis of separation of different DNA fragments by gel electrophoresis?
a. The positive charge on DNA
b. The size of the DNA fragments
c. The sequence of the fragments
d. The presence of a dye
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Students have asked these similar questions
What feature of a DNA fragment causes it to move through a gel during electrophoresis? a. the electrical charges of its phosphate groups b. its nucleotide sequence c. the hydrogen bonds between its base pairs d. its double helix shape
What is the purpose of Bromophenol Blue in electrophoresis?
a.
To follow the progress of the gel as it is running
b.
It acts as a DNA size ladder or DNA size standards.
c.
To allow the electric current to move through the gel.
d.
To visualize the DNA after the gel has finished running.
What is the purpose of acetate in TAE?
a.
It helps to maintain the pH of the buffer
b.
To carry electrons to allow the electric current to travel through the gel.
c.
It is used to stop the action of DNases
d.
It is required to see the DNA on a UV light.
Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?
A.
DNA is positively charged to attracted to the negative end of the unit
B.
the agarose gel is positively charged
C.
the agarose gel in negatively charged
D.
DNA is negatively charged so attracted to the positive end of the unit
Chapter 17 Solutions
BIOLOGY
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 2LOCh. 17.1 - Describe the construction and uses of recombinant...Ch. 17.2 - Relate the process of DNA replication to PCR.Ch. 17.2 - Compare and contrast PCR, RT-PCR, and quantitative...Ch. 17.3 - Prob. 1LOCh. 17.3 - Prob. 2LOCh. 17.3 - Describe the pros and cons of RNA interference and...Ch. 17.4 - Explain how the universal nature of the genetic...Ch. 17.4 - Compare and contrast knockout, knockin, and...
Ch. 17.4 - Prob. 3LOCh. 17.5 - Describe the benefits of biofuel production from...Ch. 17.5 - Prob. 2LOCh. 17.5 - Prob. 3LOCh. 17.6 - Prob. 1LOCh. 17.6 - Compare and contrast FISH and gene chip...Ch. 17.6 - Describe how immunoassays can be used to diagnose...Ch. 17.7 - Describe the benefits of creating transgenic...Ch. 17.7 - Prob. 2LOCh. 17.7 - Evaluate issues on each side of the transgenic...Ch. 17 - Prob. 1DACh. 17 - Prob. 2DACh. 17 - Prob. 1IQCh. 17 - Prob. 2IQCh. 17 - You study a gene known to be important in the...Ch. 17 - What is the basis of separation of different DNA...Ch. 17 - Prob. 3UCh. 17 - FISH analysis of a breast tumor biopsy for HER2...Ch. 17 - In terms of studying gene function, what is the...Ch. 17 - The Ti plasmid of Agrobacterium usually induces...Ch. 17 - Prob. 1ACh. 17 - Which of the following statements is accurate for...Ch. 17 - Prob. 3ACh. 17 - Many human proteins, such as hemoglobin, are only...Ch. 17 - Amyloid beta is a proteolytic product of a protein...
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- How are DNA fragments separated on an agarose gel duning electrophoresis ? A. The electric current makes the fragments migrate through the geThe smaller fragments migrate through the gel the fastest. B. The heavier fragments fall faster than the lighter ones and therefore, reach the end of the gel first C. The fragments are sorted based on the shape of their folding The more complicated the folded shape is, the slower the travel rate is. D. The larger fragments have more charge and, therefore, move through the gel faster than the smaller fragments John Doe has two possible fathers. The DNA fragment sizes of John Doe, John Doe's mother, a possible fathers are as follows: John Doe's mother - 850 bp and 400 bp John Doe - 680 bp and 400 bp Possible father 1 - 680 bp and 500 bp Possible father 11 - 680 bp and 280 bp Which, If either of the two men could be John Doe’s father and why? A. both of the two men, because they and John Doe all have a 680 bp sized DNA fragment B.…arrow_forwardWhat is the effect of ionizing radiation on DNA? a. faster replication b. the formation of nucleotide dimers c. kinks in the DNA molecule d. breaks in the DNA molecule e. base pair mismatchingarrow_forwardWhat is the basis for the difference in how the leading and lagging strands of DNA molecules are synthesized? a. The origins of replication occur only at 5'end b. Helicases and single-strand binding proteins work at the 5'end c. DNA polymerase can join new nucleotides only to the 3' end of a pre-existing strand d. DNA ligase works only in the 3'-5' directionarrow_forward
- Which DNA fragment will be closest to the top (negative pole) of anelectrophoretic gel?a. 450 bp b. 3,560 bp c. 5 kb d. 1,500 bparrow_forwardWhat is the correct sequence action of proteins during the process of DNA replication? A. DNA polymerase, helicase, primase, ligase, SSB B. helicase, SSB, primase, ligase, DNA polymerase C. ligase, helicase, SSB, primase, DNA polymerase D. helicase, SSB, primase, DNA polymerase, ligasearrow_forwardWhat is the major role of DNA polymerase in the DNA replication? a. Attached RNA primers to initiate the addition of complementary nucleotide sequence at the 5’ to 3’direction b. Adds up new complementary nucleotide sequence at the 3’ to 5’ direction of the DNA template. c. Unwinds the double helix by breaking the bonds at the 3’ to 5’ polymerization activity. d.It transiently cuts each strand to prevent supercoil at the 5’ to 3’ polymerization activity complementary to the RNA strandarrow_forward
- What is the role of Sucrose in DNA isolation? A.Cell Lysis B. Maintain osmotic pressure C. Maintain ionic strength D. Precipitate proteinsarrow_forwardWhich part of the DNA is broken in a double strand break? a. Hydrogen bonds between bases b. Covalent bonds between the base and the sugar c. Covalent bonds between the base and the 2'-OH group d. Covalent bonds between the 3'OH of one nucleotide and the 5' phosphate of the next nucleotidearrow_forwardWhen two adjacent bases in the same strand of DNA dimerize (form a covalent bond between them), what happens to the DNA? a. the original strand of DNA now contains a new DNA sequence b. the original strand of DNA is prevented from opening during replication, so this section of DNA will not be replicated c. the original strand of DNA is methylated, which causes the bases to mismatch d. the original strand of DNA is kinked, which prevents DNA polymerase from working properly e. the original strand of DNA is unaffected, so no additional mutations arisearrow_forward
- Why are mutations more likely to occur in repeated DNA sequences? a. These bases are unstable b. bases in the strand can form base pairs, generating loops that interfere with replication and repair enzymes. c. The repeats hold onto the replication enzymes, causing base mismatches d. the repeats attract and bind to mutagens, increasing the mutation ratearrow_forwardWhat is DNA polymerase? a.An enzyme that carries out DNA replication b.Short, single strand of DNA that base-pairs with a specific DNA sequence c.An enzyme that corrects mutations that arise during the replication of DNA d.An enzyme that seals any gaps that remain between bases of replicating strands of DNAarrow_forwardHow do amino acids in DNA-binding proteins interact with DNA? a. By forming covalent bonds with DNA bases b. By forming hydrogen bonds with DNA bases c. By forming covalent bonds with DNA sugarsarrow_forward
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