Concept explainers
To review:
The reason for use of wide host range in RDT (recombinant DNA or deoxyribonucleic acid technology) for the transformation of recombinant DNA into a bacterial cell.
Introduction:
RDT involves gene cloning and genetic engineering process, in which manipulation of the genetic material of an organism is carried out. It is done by inserting the gene of interest in the vector, which carries it to a specific host to produce multiple new genetic combinations with the help of RDT. There are many tools that are used in the gene cloning experiment, such as restriction endonucleases, DNA ligases, foreign DNA or DNA insert, vector, and the host cell. Moreover, the host cell supports the amplification of the DNA fragment for gene expression at the
Want to see the full answer?
Check out a sample textbook solutionChapter 17 Solutions
EBK ESSENTIALS OF GENETICS
- What Are Clones? Cloning is a general term used for whole organisms and DNA sequences. Define what we mean when we say we have a clone.arrow_forwardIn cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardList the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs and YACs provide over plasmids as cloning vectors?arrow_forward
- What is a cloning vector? Give two examples of specific DNA molecules routinely used as cloning vectors.arrow_forwardPLease explain What characteristics of plasmids makes them good cloning vectors? Discussarrow_forwardWhat characteristics of plasmids makes them good cloning vectors? Discuss.arrow_forward
- Following are four processes common to most cloning experiments: a) transforming bacteria b) plating bacteria on selective medium c) cutting DNA with restriction endonucleases d) ligating DNA fragments Place components of this list in the order in which they would most likely occur during a cloning experiment.arrow_forwardList and explain the steps of DNA cloning using pUC8.arrow_forwardHow can site-specific recombination be used in recombinant DNA technology? Explain with an examplearrow_forward
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage LearningBiology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage Learning