Essentials of Genetics, Books a la Carte Plus Mastering Genetics with eText -- Access Card Package (9th Edition)
9th Edition
ISBN: 9780134319070
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Textbook Question
Chapter 17, Problem 8PDQ
List the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs and YACs provide over plasmids as cloning vectors?
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List the advantages and disadvantages of using plasmids as cloning vectors. What advantages do BACs and YACs provide over plasmids as cloning vectors?
What characteristics of plasmids makes them good cloning vectors? Discuss.
What are the essential elements of a plasmid cloning vector? Discuss the importance of each element for dna cloning.
Chapter 17 Solutions
Essentials of Genetics, Books a la Carte Plus Mastering Genetics with eText -- Access Card Package (9th Edition)
Ch. 17 -
CASE STUDY |Should we worry about recombinant DNA...Ch. 17 - Prob. 2CSCh. 17 - Prob. 3CSCh. 17 -
HOW DO WE KNOW?
1. In this chapter we focused on...Ch. 17 - Prob. 2PDQCh. 17 - What roles do restriction enzymes, vectors, and...Ch. 17 - Prob. 4PDQCh. 17 - Prob. 5PDQCh. 17 - Prob. 6PDQCh. 17 - Prob. 7PDQ
Ch. 17 - List the advantages and disadvantages of using...Ch. 17 - What are the advantages of using a restriction...Ch. 17 - The introduction of genes into plants is a common...Ch. 17 - Prob. 11PDQCh. 17 - Prob. 12PDQCh. 17 - Prob. 13PDQCh. 17 - What advantages do cDNA libraries provide over...Ch. 17 - Prob. 15PDQCh. 17 -
16. List the steps involved in screening a...Ch. 17 -
17. In a typical PCR reaction, describe what is...Ch. 17 -
18. We usually think of enzymes as being most...Ch. 17 - How are dideoxynucleotides (ddNTPs) structurally...Ch. 17 - Prob. 20PDQCh. 17 - One complication of making a transgenic animal is...Ch. 17 -
22. When disrupting a mouse gene by knockout, why...Ch. 17 - Prob. 23PDQ
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What is a cloning vector? Give two examples of specific DNA molecules routinely used as cloning vectors.arrow_forwardIn cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardWhat would happen if a plasmid without a selectable marker was chosen as a cloning vector?arrow_forward
- What is an engineered plasmid used for? What are the important features of\ engineered plasmids?arrow_forwardWhat are the advantages of using organisms such as bacteria and yeast as cloning hosts? What safety considerations must be made when choosing a cloning host to make proteins for human use?arrow_forwardWhat would be an advantage of using HaeIII for a cloning experiment? What would be a disadvantage?arrow_forward
- Ampicillin and tetracycline resistant genes are sometimes located on plasmids used in cloning. What is the importance of such genes in tranformation? What is the principle involved?arrow_forwardIf the cloning was successful and the plasmid contained the elements needed for blue-white screening, what should be the color of the colonies harboring the correct plasmid?arrow_forwardWhat roles do restriction enzymes, vectors, and host cells play in recombinant DNA studies? What role does DNA ligase perform in a DNA cloning experiment? How does the action of DNA ligase differ from the function of restriction enzymes?arrow_forward
- Which vectors (plasmid, phage λ, cosmid, bacterial artificial chromosome) can be used to clone a continuous fragment of DNA with the following lengths? a. 4 kb b. 20 kb c. 35 kb d. 100 kbarrow_forwardBesides their role in genetic engineering, what else do plasmids can plasmids be used for?    How are restriction enzymes used in genetic engineering?arrow_forwardWhat types of cells are used for cloning rDNA?arrow_forward
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