Prescott's Microbiology

10th Edition

ISBN: 9781259281594

Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor

Publisher: McGraw-Hill Education

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Textbook Question

Chapter 17.3, Problem 2RIA

Why is PCR used to detect infectious agents that are often hard to diagnose?

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Chapter 17.3, Problem 1RIA

Chapter 17.3, Problem 3RIA

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Chapter 17 Solutions

Prescott's Microbiology

Ch. 17.1 - Examine the uncut piece of DNA shown in the upper...Ch. 17.1 - Which of the above enzymes yield blunt ends? Which...Ch. 17.1 - Why might two DNA fragments inadvertently be...Ch. 17.1 - Why must introns be removed from eukaryotic DNA...Ch. 17.1 - If a linear piece of DNA is cut with a restriction...Ch. 17.1 - Describe restriction enzymes, sticky ends, and...Ch. 17.1 - What is cDNA? Why is it necessary to generate cDNA...Ch. 17.1 - You want to visualize a digested plasmid that...Ch. 17.1 - What is the purpose of Southern blotting? How is a...Ch. 17.2 - Why, after three cycles, are the vast majority of...

Ch. 17.3 - Which plasmid is a shuttle vector? Why?Ch. 17.3 - What would you conclude if you obtained only blue...Ch. 17.3 - In what ways does the BAC shown here differ from...Ch. 17.3 - Briefly describe the polymerase chain reaction....Ch. 17.3 - Why is PCR used to detect infectious agents that...Ch. 17.3 - How would you use PCR to measure the concentration...Ch. 17.3 - Why is it possible to visualize a PCR product on...Ch. 17.3 - You want to clone a 6,000 bp DNA fragment in E...Ch. 17.5 - Why are long fragments (e.g., 20,000 bp) of...Ch. 17.6 - What special considerations are necessary if one...Ch. 17.6 - Prob. 1RIA Ch. 17.6 - Explain the selection for antibiotic resistance...Ch. 17.6 - Prob. 3RIA Ch. 17.6 - Prob. 4RIA Ch. 17.6 - Prob. 5RIA Ch. 17.6 - You are studying chemotaxis proteins in a newly...Ch. 17 - Which of the DNA molecules shown are recombinant?Ch. 17 - You are performing a PCR to amplify a gene...Ch. 17 - Prob. 2CHI Ch. 17 - Suppose you transformed a plasmid vector carrying...Ch. 17 - You are interested in the activity and regulation...Ch. 17 - Prob. 5CHI Ch. 17 - Zymomonas mobilis is a Gram-negative bacterium...

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Why is PCR used to detect infectious agents that are often hard to diagnose?

Solution Summary: The author explains that polymerase chain reaction (PCR) was designed by Kary Mullis in the year 1980. It is used to amplify the small amount of particular DNA sequences.

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Chapter 17 Solutions