Chapter 18, Problem 18.70P

Interpretation Introduction

Interpretation:

The initial rate of the reaction and the value of $R_{\max }$ have to be calculated.

Concept Introduction:

Lineweaver – Burk equation:

The Lineweaver – Burk equation is given below.

  $\begin{array}{l}R=\frac{R_{\max }\left[S\right]}{\left[S\right]+K_M}\\ \\ \frac{1}{R}=\left(\frac{K_M}{R_{\max }}\right)\left(\frac{1}{\left[S\right]}\right)+\frac{1}{R_{\max }}\end{array}$

Where, $R_{\max }$ is the maximum rate of a reaction, $K_M$ is Michaelis – Menten constant and $\left[S\right]$ is the substrate concentration.

The turnover number can be estimated as given below.

  $Turnovernumber=\frac{R_{\max }}{{\left[E\right]}_t}=k_2$