BROCK BIOL OF MICRO-ACCESS W/EBOOK
4th Edition
ISBN: 9781323632215
Author: MADIGAN
Publisher: PEARSON C
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Chapter 18, Problem 1AQ
Design an experiment for measuring the activity of sulfuroxidizing bacteria in soil. If only certain species of the sulfur oxidizers present were
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Chapter 18 Solutions
BROCK BIOL OF MICRO-ACCESS W/EBOOK
Ch. 18.1 - Describe the enrichment strategy behind...Ch. 18.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 18.1 - What is enrichment bias? How does dilution reduce...Ch. 18.2 - How does the agar dilution method differ from...Ch. 18.2 - How might you isolate a morphologically unique...Ch. 18.2 - What is meant by high-throughput in culturing...Ch. 18.3 - How does viability staining differ from stains...Ch. 18.3 - Prob. 2MQCh. 18.3 - Why is it incorrect to say that the GFP is a...Ch. 18.4 - What structure in the cell is the target for...
Ch. 18.4 - FISH and CARD-FISH can be used to reveal different...Ch. 18.5 - What could you conclude from PCR/DGGE analysis of...Ch. 18.5 - What surprising finding has come out of many...Ch. 18.6 - MINIQUIZ
• What is a phylochip and what can it...Ch. 18.6 - What are the advantages and disadvantages of...Ch. 18.6 - Prob. 3MQCh. 18.7 - Prob. 1MQCh. 18.7 - How do environmental genomic approaches differ...Ch. 18.7 - Prob. 3MQCh. 18.8 - Prob. 1MQCh. 18.8 - If a large pulse of organic matter entered the...Ch. 18.9 - Prob. 1MQCh. 18.9 - What is the simplest explanation for why lunar...Ch. 18.9 - What is the expected isotopic composition of...Ch. 18.10 - How could NanoSIMS be used to identify a...Ch. 18.10 - Prob. 2MQCh. 18.10 - How does MAR-FISH link microbial diversity and...Ch. 18.11 - How can stable isotope probing reveal the identity...Ch. 18.11 - What key method is required to do genomics on a...Ch. 18.11 - Prob. 3MQCh. 18 - Prob. 1RQCh. 18 - Prob. 2RQCh. 18 - Prob. 3RQCh. 18 - Prob. 4RQCh. 18 - Prob. 5RQCh. 18 - Prob. 6RQCh. 18 - Prob. 7RQCh. 18 - Prob. 8RQCh. 18 - Prob. 9RQCh. 18 - REVIEW QUESTIONS
10. Why is a microarray not...Ch. 18 - Prob. 11RQCh. 18 - Prob. 12RQCh. 18 - Q What are the major advantages of radioisotopic...Ch. 18 - Prob. 14RQCh. 18 - Prob. 15RQCh. 18 - REVIEW QUESTIONS
16. What is the advantage of...Ch. 18 - Prob. 17RQCh. 18 - Design an experiment for measuring the activity of...Ch. 18 - You wish to know whether Archaea exist in a lake...Ch. 18 - Design an experiment to solve the following...Ch. 18 - Design a SIP experiment that would allow you to...
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- Can you show in a diagram how sulfur is cycled (Sulfur cycle) in a water column (Winogradsky column) starting from sulfate? Please indicate the name of the important metabolic pathways and physiological groups involved.arrow_forwardIn one final experiment, you add all the necessary components and program the thermocycler to cycle under the following temperatures Denaturation 95 degrees, 30 seconds, Annealing 65 degrees, 30 seconds, Elongation 68 degrees 30 seconds You find out that the third time's the charm, but now you have many small incomplete fragments instead. What happened and how did you fix it?arrow_forwardFermentation is a means of food preservation. What end products produced in fermentation are antagonistic or inhibitory to spoilage organisms?arrow_forward
- Using supporting calculations, determine the energy and cost (carbon) savingsassociated with the following biological nitrogen removal strategy: Partial nitrification (oxidation of all the influent ammonia to nitrite) and partialdenitrification (reduction of produced nitrite to nitrogen gas)arrow_forwardIn an experiment where you use different concentrations of avocado catalase to determine how that affects rate of the reaction with its substrate, hydrogen peroxide, why was the amount(concentration) of peroxide kept the same for all tubes? Why was the total volume of every tube 10cm?arrow_forwardName a high energy phosphate compound capable of substrate level phosphorylation?arrow_forward
- For an experiment where you use different concentrations of catalase to determine how that affects rate of the reaction with its substrate, hydrogen peroxide, what effect does enzyme concentration have on reaction activity? Would it be possible to have too much catalase in the tube? What would happen?arrow_forwardWhat is the metabolic advantage of a substrate cycle? What is its cost?arrow_forwardYou are attempting to determine KM by measuring the reaction velocity at different substrate concentrations, but you do not realize that the substrate tends to precipitate under the experimental conditions you have chosen. How would this affect your measurement of KM?arrow_forward
- Define the following terms: (1) bioenergetics, (2) ender- gonic reactions, and (3) exergonic reactions.arrow_forwardClassify each of the following molecules as (1) an oxidizing agent, (2) a reducing agent, or (3) neither an oxidizing agent nor a reducing agent. a. FAD b. ADP c. Coenzyme A d. NAD+arrow_forwardTo identify the molecule that accepts CO2, Calvin and Benson manipulated the carbon-fixation cycle by either cutting off CO2 or cutting off light from cultures of photosynthetic algae. They then measured the concentrations of various metabolites immediately following the manipulation. How would these experiments help identify the CO2 acceptor? Select one: a. The CO2 acceptor concentration would decrease when the CO2 is cut off, but increase when the light is cut off. b. The CO2 acceptor concentration would increase when the CO2 is cut off, but decrease when the light is cut off. c. The CO2 acceptor concentration would decrease when either the CO2 or light are cut off. d. The CO2 acceptor concentration would increase when either the CO2 or light are cut off.arrow_forward
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