CAMPBELL BIOLOGY IN FOCUS-TEXT,AP ED.
3rd Edition
ISBN: 9780136811206
Author: Urry
Publisher: SAVVAS L
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Chapter 18.3, Problem 2CC
Summary Introduction
To describe:
The information given on the website: https://gold.jgpi-psf.org/statistics and also to give percent of bacterial genome projects that have medical relevance.
Introduction:
The genome online database (GOLD) is a website of Joint Genome Institute that gives information about genome sequencing projects.
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What exactly is the purpose of the programme Finch TV? Examining the results of sequencing of the next generation It is necessary to use the.bam file extension in order to access sequencing files. For sanger sequencing files, the.ab1 file extension is required in order to open them. It is possible to open NGS files by using the.ab1 file extension.
Place the steps necessary to perform RNA-Seq in the correct order.
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correct order.
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MAAAAAAAAAKK
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Compare sequences to known genome sequence.
Create cDNA using reverse transcription with primers
complementary to linkers.
Attach linkers to the ends of the RNAs.
Perform next-generation DNA sequencing.
Isolate RNA from cells or tissues of interest.
Fragment the RNAs.
Ihsan is a biologist working with the genetics of a psychrophilic bacterium. He cloned an
antifreeze gene from the bacteria for further analysis. After cloning, he isolated the plasmid
carrying his gene of interest for sequencing. Ihsan finally received the nucleotide sequence of
his gene. Explain in detail how he could verify whether the nucleotide sequence matches his
gene of interest using the bioinformatics databases available.
Chapter 18 Solutions
CAMPBELL BIOLOGY IN FOCUS-TEXT,AP ED.
Ch. 18.1 - Prob. 1CCCh. 18.2 - Prob. 1CCCh. 18.2 - Explain the advantage of the systems biology...Ch. 18.2 - Prob. 3CCCh. 18.3 - The best estimate is that the human genome...Ch. 18.3 - Prob. 2CCCh. 18.3 - Prob. 3CCCh. 18.4 - Discuss the characteristics of mammalian genomes...Ch. 18.4 - Which of the three mechanisms described in Figures...Ch. 18.4 - Prob. 3CC
Ch. 18.5 - Describe three examples of errors in cellular...Ch. 18.5 - Prob. 2CCCh. 18.5 - Prob. 3CCCh. 18.6 - Would you expect the genome of the macaque (a...Ch. 18.6 - Prob. 2CCCh. 18 - Prob. 1TYUCh. 18 - Prob. 2TYUCh. 18 - Two eukaryotic proteins have one domain in common...Ch. 18 - DRAW IT Comparing amino acid sequences of similar...Ch. 18 - SCIENTIFIC INQUIRY The scientists mapping human...Ch. 18 - FOCUS ON EVOLUTION Genes important in the...Ch. 18 - FOCUS ON INFORMATION The continuity of life is...Ch. 18 - SYNTHESIZE YOUR KNOWLEDGE Insects have three...
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- Hi, I would like to know which program is used for the graphical presentation of the results of a meta-analysis of genome-wide linkage scans?arrow_forwardJackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned a heat shock gene from the bacteria for further analysis. After cloning, he isolated the plasmid carrying his gene of interest for sequencing. Jackson finally received the nucleotide sequence of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his gene of interest using the bioinformatics databases available.arrow_forwardWhat advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.arrow_forward
- You need to complete the following steps for an experiment. Explain whether an acrylamide gel or an agarose gel is more appropriate to use for the following experiments. Purifying tRNA from total RNA Isolating a vector insert for cloning Analyzing PCR productsarrow_forwardwhat is the main purpose of performing the bioinformatics analysis of 16s rRNA genes lab?arrow_forwardAs the leading scientist in a biomedical science laboratory, it is a requirement to give advice to your lab assistants when they are having problems with their experiments. What advice would you give to your assistants that are having the following problems: After performing a polymerase chain reaction (PCR) and agarose gel electrophoresis to confirm the presence of the C01 gene of 750bp. 2.1. They observe no band appearing on an agarose gel. What would be your conclusion? 2.2. They observe three bands of different sizes that resemble a smear on the gel. Advice 2.3. They observe a single band on the gel and conclude that the PCR product is an exact copy of the original template DNA. Would you support their condusion? Explain. 2.4. Explain how PCR can be used to detect infectious agents in diagnoses of diseases.arrow_forward
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