Gen Combo Ll Biology; Connect W/learnsmart Labs Access Card
4th Edition
ISBN: 9781259853197
Author: Robert Brooker
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 18.5, Problem 1BC
Gene Transfer Between Bacteria
Core Skill: Connections Look back at Figures 11.1 and 11.2. How did the phenomenon of transformation allow researchers to demonstrate that DNA is the genetic material?
Figure 11.1 Griffith’s experiments showing that genetic material can be transferred from one bacterium to another. Note: To determine if a mouse’s blood contained live bacteria, a sample of blood was also applied to solid growth media. (This part of the procedure is not shown.) For steps 1 and 4, smooth bacterial colonies were observed. For step 2, no bacterial colonies were observed because the type R cells were killed by the immune system of the mouse.
Figure 11.2 The Avery, MacLeod, and McCarty experiments that identified DNA as Griffith’s transformation principle—the genetic material.
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TOPIC: PCR and Gene Cloning Basics
Question: What are 2 possible roles of CaCl2 in the transformation process?
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Polymerase Chain Reaction (PCR) (Links to an external site.) is an essential technique for the study of DNA. PCR uses a DNA polymerase enzyme from Thermophilus aquaticus (Links to an external site.) to amplify DNA extracted from any sample. In order for the reaction to work, researchers must transfer microliters of sample to the reaction vessel for PCR.
If a researcher has a DNA sample with a concentration of 0.2 micrograms per microliter, how many microliters of DNA must be transfered to conduct a 50 microliter reaction if the final concentration should be 2.3 nanograms per microliter?
Report your answer to two decimal places using standard decimal notation (e.g. 0.5, not 5.0e-1)
explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. (1) Restriction Fragment Length Polymorphism (RFLP)(2) Pulsed-Field Gel Electrophoresis (PFGE)(3) Whole Genome Sequencing (WGS)(4) Gram Staining(5) Biochemical Reactions: Multiple Tube Fermentation Technique(6) Biochemical Reactions: IMViC Test
Chapter 18 Solutions
Gen Combo Ll Biology; Connect W/learnsmart Labs Access Card
Ch. 18.1 - Prob. 1CCCh. 18.2 - Prob. 1BCCh. 18.2 - Viral Reproductive Cycles Concept Check: From the...Ch. 18.4 - Prob. 1CCCh. 18.4 - Genetic Properties of Bacteria Core Skill:...Ch. 18.4 - Prob. 2CCCh. 18.4 - Genetic Properties of Bacteria Concept Check:...Ch. 18.4 - Prob. 4CCCh. 18.5 - Prob. 1CCCh. 18.5 - Prob. 1EQ
Ch. 18.5 - Prob. 2EQCh. 18.5 - Gene Transfer Between Bacteria CoreSKILL The gene...Ch. 18.5 - Prob. 2CCCh. 18.5 - Gene Transfer Between Bacteria Core Skill:...Ch. 18.5 - Gene Transfer Between Bacteria Concept Check: Is...Ch. 18 - Prob. 1TYCh. 18 - The characteristics of viral genomes show many...Ch. 18 - During viral infection, attachment is usually...Ch. 18 - Prob. 4TYCh. 18 - Prob. 5TYCh. 18 - Prob. 6TYCh. 18 - Prob. 7TYCh. 18 - Prob. 8TYCh. 18 - Prob. 9TYCh. 18 - Prob. 10TYCh. 18 - How are viruses similar to living cells, and how...Ch. 18 - Prob. 2CQCh. 18 - Prob. 3CQCh. 18 - Prob. 1COQCh. 18 - Prob. 2COQ
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