Interpretation:
The precautions that must be taken when the mixture of compounds is introduced onto the liquid chromatography adsorbent column should be explained.
Concept introduction:
Liquid chromatography is used for purification of high boiling compounds. It is known as column chromatography. In liquid chromatography, column is packed by stationary phase. Usually a solid adsorbent with a liquid coated on it is used as stationary phase. Often a pure liquid or solution of liquids compose the mobile phase. It is also called the elution solvent.
The elution solvent comes down the column under the influence of gravity. Selective interactions are responsible for separation of the sample among stationary and mobile phase. The sequence of elution of compounds from sample depends on the polarity of mobile and stationary phase.
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Chapter 19 Solutions
LABORATORY TECHNIQUES IN ORGANIC CHEMIS
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