EBK BROCK BIOLOGY OF MICROORGANISMS
15th Edition
ISBN: 8220103633352
Author: Stahl
Publisher: PEARSON
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Chapter 19.12, Problem 1MQ
- How can stable isotope probing reveal the identity of an organism that carries out a particular process?
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Determine the methods that could be used on a sample (obtained from the ocean of Jupiter's moon) to differentiate between a novel cellular life form and contamination of an organism from the Earth based on DNA, RNA, and protein.
Define ecotype. Do you think it is necessary to obtain microbes in pure culture before assigning different ecotypes? Explain.
What is meant by the term “enrichment culture”?
Chapter 19 Solutions
EBK BROCK BIOLOGY OF MICROORGANISMS
Ch. 19.1 - Describe the enrichment strategy behind...Ch. 19.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 19.1 - What is enrichment bias? How does dilution reduce...Ch. 19.1 - Why do the results of a direct enrichment of an...Ch. 19.2 - What is a pure culture and why is obtaining one...Ch. 19.2 - How does the agar dilution method differ from...Ch. 19.2 - What criteria serve to demonstrate that a culture...Ch. 19.3 - How might you isolate a morphologically unique...Ch. 19.3 - What is meant by high-throughput in culturing...Ch. 19.3 - What feature of high-throughput culturing relieves...
Ch. 19.4 - How does viability staining differ from stains...Ch. 19.4 - What types of environments limit the application...Ch. 19.4 - Why is it incorrect to say that the GFP is a...Ch. 19.4 - Prob. 1CRCh. 19.5 - What structure in the cell is the target for...Ch. 19.5 - FISH and CARD-FISH can be used to reveal different...Ch. 19.5 - Why is CARD-FISH more suitable than FISH for...Ch. 19.6 - What could you conclude from PCR/DGGE analysis of...Ch. 19.6 - What surprising finding has come out of many...Ch. 19.6 - How has next-generation sequencing technology...Ch. 19.6 - QWhich method, ARISA or T-RFLP, would provide more...Ch. 19.7 - Prob. 1MQCh. 19.7 - What are the advantages and disadvantages of...Ch. 19.7 - Why might a microarray be superior to using...Ch. 19.8 - Prob. 1MQCh. 19.8 - How do environmental genomic approaches differ...Ch. 19.8 - Prob. 3MQCh. 19.8 - Prob. 1CRCh. 19.9 - Prob. 1MQCh. 19.9 - If a large pulse of organic matter entered the...Ch. 19.9 - Q What are the major advantages of radioisotopic...Ch. 19.10 - What is the simplest explanation for why lunar...Ch. 19.10 - What is the expected isotopic composition of...Ch. 19.10 - How might exchange of metabolites among members of...Ch. 19.10 - Will autotrophic organisms contain more or less...Ch. 19.11 - How could NanoSIMS be used to identify a...Ch. 19.11 - Prob. 2MQCh. 19.11 - How does MAR-FISH link microbial diversity and...Ch. 19.11 - Q What can MAR-FISH tell you that FISH alone...Ch. 19.12 - How can stable isotope probing reveal the identity...Ch. 19.12 - What key method is required to do genomics on a...Ch. 19.12 - Prob. 3MQCh. 19.12 - How would you use cytometric cell sorting to...Ch. 19 - Design an experiment for measuring the activity of...Ch. 19 - You wish to know whether Archaea exist in a lake...Ch. 19 - Design an experiment to solve the following...Ch. 19 - Design a SIP experiment that would allow you to...
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- What is the basis of enrichment culture techniques? Why is an enrichment medium usually suitable for the enrichment of only a certain group or groups of organisms?arrow_forwardExplain, in your own words, why it is important to perform serial dilution when trying to quantify bacterial organisms.arrow_forwardDetermine the approaches that might be tried on a sample (obtained from ocean of Jupiter's moon) to distinguish between contamination of an organism from the Earth, and a novel cellular life-form on the basis of DNA, RNA, and protein. Don't reroute.arrow_forward
- what was the driving force for the development of microscale testingmethods for ecotoxicology.arrow_forwardDescribe how would you determine the following using a real-time PCR instrument. A) The quantity and quality of a DNA sample. B) Whether an individual had cystic fibrosis from a genomic DNA sample. C) If several bacterial pathogens were present in DNA extracted from a food sample.arrow_forwardBriefly describe an irreversible and a reversible hypotheses to explain the loss of morphogenic potential for in vitro cultures.arrow_forward
- Using the clinical question --"Does antibacterial foam decrease bacteria count on hands as much as hand washing with soap and water.” Question 1) Describe your study design and sample. 2) Will your findings be generalizable? Why or why not? 3) How does this exercise inform your interpretation of published research?.arrow_forwardHow reliable is PCR to detect microorganisms in water samples?arrow_forwardCan gel electrophoresis be a quantitative technique? How so?arrow_forward
- Which of the following types of data produce curves similar in shape to the graph? Select all that apply 1.Bacterial growth in a culture 2.A normal distribution of height in a population of students 3.Exponential population growth 4.Haemoglobin binding of oxygen 5.Log transformations of drug dose curves 6.Osmotic fragility of red blood cells 7.A standard curve for an enyme assayarrow_forwardDefine the following termonologies in brief 1.Cellular Permeability 2.Fluorescence in situ Hybridization(FISH) 3. Genotoxicity 4.Medical Device 5. Sterilizationarrow_forwardMannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?arrow_forward
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