The use of probes in screening DNA libraries. Introduction: DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

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Answer 1

Question

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

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Answer 2

Question

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Expert Solution & Answer

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Answer 3

Question

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Expert Solution & Answer

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Answer 4

Question

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Expert Solution & Answer

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See solution

Answer 5

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Answer 6

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Answer 7

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Answer 8

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Answer 9

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 19.1 - Prob. 1COQ Ch. 19.1 - Prob. 1TPSQ Ch. 19.2 - Prob. 1CC Ch. 19.2 - Prob. 2CC Ch. 19.2 - Prob. 3CC Ch. 19.2 - Prob. 2COQ Ch. 19.2 - Prob. 3COQ Ch. 19.2 - Prob. 4COQ Ch. 19.2 - Prob. 5COQ Ch. 19.2 - Prob. 6COQ

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Chapter 19 Solutions