Prescott's Microbiology
11th Edition
ISBN: 9781260409062
Author: WILLEY, Joanne
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 19.5, Problem 3CC
Of the following genes, which do you think are part of the pangenome and which are part of the core genome: the genes for lactose catabolism in E coli; the genes for heat-stable DNA polymerase in Thermus aquaticus; the genes for proteorhodopsin in marine bacteria; the genes for toxin production in Vibrio cholerae?
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Which of the following statements are true regarding the metabolome, the proteome, the genome, and the transcriptome?
The number of genes found in the genome equals the number of different proteins that can be made within any give cell.
The number of transcription products equals the number of proteins that can be made from these transcription products.
The number of genes found in the genome equals the number of different transcription products that can be made within any given cell.
The metabolome is encoded by the genome.
None of the above.
The figure below shows the life cycle of the fungus Neurospora. The adult stage of the Neurospora is a multicellular haploid.
b) Neurospora has an arginine amino acid synthesis pathway shown below. Suppose I take the strain above that only grows with arginine supplements and cross it to a different mutant Neurospora strain that grows with arginine and citrulline supplements but not with ornithine supplements. Assuming gens A, B, and C are unlinked and there is only one mutation per stain:
What percentage of the progeny will grow on ornithine?
What percentage on citrulline?
What percentage on arginine?
Genetic analysis of Mycobacterium leprae, the bacterium that causes leprosy, reveals that its genome has undergone decay over time, losing DNA and acquiring mutations that make some of its genes nonfunctional. What might be some potential reasons for this evolutionary decay of its genome?
Chapter 19 Solutions
Prescott's Microbiology
Ch. 19.1 - What is a natural classification? What microbial...Ch. 19.1 - Prob. 2CCCh. 19.1 - Consider the finding that bacteria capable of...Ch. 19.2 - What is the difference between a microbial species...Ch. 19.2 - Why is it important to have a type strain for each...Ch. 19.2 - The genus Salmonella was once thought to contain...Ch. 19.3 - What are the advantages of using each major group...Ch. 19.3 - Why is rRNA so suitable for determining...Ch. 19.3 - Prob. 3CCCh. 19.4 - Could a phylotype be considered an OTU? What about...
Ch. 19.4 - List the differences between distance-based and...Ch. 19.4 - Prob. 3CCCh. 19.4 - You are building a tree based on 16S rRNA sequence...Ch. 19.4 - Is HGT involved in movement of genes in the core...Ch. 19.5 - On what evidence is this hypothesis based?Ch. 19.5 - Construct a scenario in which each of the...Ch. 19.5 - Define ecotype. Do you think it is necessary to...Ch. 19.5 - What is the difference between the core genome and...Ch. 19.5 - Of the following genes, which do you think are...Ch. 19.5 - Prob. 4CCCh. 19.6 - Prob. 1CCCh. 19.6 - Describe two different situations in which it...Ch. 19 - Prob. 1RCCh. 19 - Prob. 2RCCh. 19 - Prob. 3RCCh. 19 - Prob. 4RCCh. 19 - Prob. 5RCCh. 19 - Prob. 6RCCh. 19 - Consider the fact that the use of 16S rRNA...Ch. 19 - You have recently established a pure culture of a...Ch. 19 - Prob. 3ALCh. 19 - Prob. 4ALCh. 19 - Prob. 5ALCh. 19 - Prob. 6ALCh. 19 - Prob. 7AL
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- Researchers systematically replaced 414 essential genes in yeast with similar genes from humans. Almost half of these transplants (47%) were successful: cells with the humanized gene were able to function and grow. What does this observation tell us about differences between yeast and humans? How might this information be used?arrow_forwardAntibiotics such as chloramphenicol, tetracycline, and erythromycin inhibit protein synthesis in bacteria, but have no effect on the synthesis of proteins encoded by eukaryotic nuclear genes. Cycloheximide inhibits the synthesis of proteins encoded by nuclear genes, but has no effect on bacterial protein synthesis. How might these compounds be used to determine which proteins are encoded by mitochondrial and chloroplast genomes?arrow_forward. Figure 1-7 shows a simplified pathway for argininesynthesis in Neurospora. Suppose you have a specialstrain of Neurospora that makes citrulline but not arginine. Which gene(s) are likely mutant or missing inyour special strain? You have a second strain ofNeurospora that makes neither citrulline nor argininebut does make ornithine. Which gene(s) are mutant ormissing in this strain?arrow_forward
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- In humans, the AMY1 gene produces the enzyme amylase in cells of the salivary glands. Amylase breaks down starch (a polysaccharide) into the sugar maltose (a disaccharide). People from cultures with diets high in starch produce more amylase than people from cultures with diets low in starch because of a mutation in the AMY1 gene. Explain in two to three sentences why the frequency of this AMY1 mutation would have increased in frequency in populations with a high starch diet.arrow_forwardImagine that there is an E. coli outbreak in your area, and you would like to test the kangkong from your local grocery store. How could you modify this protocol to extract DNA from the kangkong (to identify the species) and check for presence or absence of E. coli.? Keep in mind that (i) E. coli is free-living and not an endosymbiont, and (ii) plant cells are encased in both a cell membrane and cell wall.arrow_forwardWhich genomes are larger, those of chloroplasts orthose of mitochondria? How does your genome comparewith that of yeast in overall size and gene number?arrow_forward
- Which vitamin is lacking from rice? Describe how genetic engineering has addressed this problem. What is the name of the GMO rice, and is it now widely cultivated?arrow_forwardDomestic dogs are closely related to wolves. Wolves have one copy of the gene that encodes the enzyme amylase. Amylase is required to digest starch. Dogs have between 4 and 30 copies of this gene. Explain how the new copies arose in the genome of the domestic dog. Compare this to the evolution of lactase persistence in humans. Explain how the diet and environment of a dog compared to a wolf would result in fixation of the duplicated gene. What possible explanation is there for the variation of copy number among dog breeds?arrow_forwardHow do you know if the halibut you purchased at the supermarket is really halibut? To identify the source of a biological sample, scientists PCR amplify and then sequence a region of DNA known to vary between species. For animals, this DNA region is a 648-base pair portion of the mitochondrial cytochrome oxidase I gene. The sequence of this mtDNA region acts as a so-called DNA barcode because a database exists that contains the sequences of this mtDNA region that are unique for hundreds of thousands of animal species. A) Why do you think that a region of mitochondrial DNA is used for barcoding animals, as opposed to a region of nuclear genomic DNA?arrow_forward
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